FastQCFastQC Report
Thu 26 May 2016
SRR1512661_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512661_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1899537
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT80330.4228925259155258No Hit
GGTATCAACGCAGAGTACTTTTTTT55870.29412430502801473No Hit
TATCAACGCAGAGTACTTTTTTTTT54810.28854399782683887No Hit
GTCCTAAAGTGTGTATTTCTCATTT30250.15924933286374524No Hit
CTGTAGGACGTGGAATATGGCAAGA29640.15603802400269118No Hit
GTCCTACAGTGGACATTTCTAAATT29540.15551157992710854No Hit
CTTTAGGACGTGAAATATGGCGAGG27260.14350865500382462No Hit
ACGCAGAGTACTTTTTTTTTTTTTT25930.13650694879857567No Hit
GTACTTTTTTTTTTTTTTTTTTTTT21500.1131854762502652No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGCGC751.4824096E-512.66382310
TAGGACC4350.011.80044
GGACCGT600.005881685811.0820136
ACCCGTG600.005885145611.0811387
GGTATCA16000.010.9482051
TAGGACA4900.010.863864
TAGGACT3050.010.5967154
GGCGAGG8550.010.21669919
GTATCAA31850.010.16282751
TGGCGAG17900.010.07861618
GTGCTAG2002.3646862E-119.9961871
CGAAATC2002.5465852E-119.9717113
GTCCTAA9500.09.8208161
TGTAGGA17750.09.7484122
GTAGGAC17650.09.7482373
GTATAAG2159.094947E-129.7415781
ACGAAAT2054.1836756E-119.7290112
ACACCGA800.00452066969.4988686
AAGTCGT1804.1945896E-99.4981177
GTCCTAC15100.09.4571311
Produced by FastQC (version 0.11.5)