##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512661_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1899537 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.71597499811796 33.0 31.0 34.0 30.0 34.0 2 31.846085124954133 34.0 31.0 34.0 30.0 34.0 3 31.944323274566383 34.0 31.0 34.0 30.0 34.0 4 35.3932068709375 37.0 35.0 37.0 33.0 37.0 5 35.16837997891065 37.0 35.0 37.0 33.0 37.0 6 35.023509413083296 37.0 35.0 37.0 32.0 37.0 7 35.016339771217936 37.0 35.0 37.0 32.0 37.0 8 35.01870876955805 37.0 35.0 37.0 32.0 37.0 9 36.62524604679982 39.0 37.0 39.0 32.0 39.0 10 36.43591306723691 39.0 37.0 39.0 32.0 39.0 11 36.54059120722576 39.0 37.0 39.0 32.0 39.0 12 36.48707764049871 39.0 37.0 39.0 32.0 39.0 13 36.48261813273445 39.0 37.0 39.0 32.0 39.0 14 37.74624763824026 40.0 38.0 41.0 32.0 41.0 15 37.726415963468995 40.0 38.0 41.0 32.0 41.0 16 37.69614806134337 40.0 38.0 41.0 32.0 41.0 17 37.66727049802136 40.0 38.0 41.0 32.0 41.0 18 37.59665855416346 40.0 38.0 41.0 32.0 41.0 19 37.62908171833452 40.0 38.0 41.0 32.0 41.0 20 37.58959104244877 40.0 38.0 41.0 32.0 41.0 21 37.54835309867615 40.0 38.0 41.0 32.0 41.0 22 37.444244044733004 40.0 38.0 41.0 32.0 41.0 23 37.39308368302381 40.0 38.0 41.0 32.0 41.0 24 37.34315783267186 40.0 38.0 41.0 32.0 41.0 25 37.27445846014055 40.0 38.0 41.0 32.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.4443820839668149 1101 2 0.40245722436023 1101 3 0.38573112408314714 1101 4 0.4048951460273287 1101 5 0.47783128554613086 1101 6 0.6212614201148554 1101 7 0.6238752960753331 1101 8 0.6506561279039005 1101 9 0.7012311909251849 1101 10 0.7730082950101647 1101 11 0.8048101135941863 1101 12 0.7480139088817808 1101 13 0.7229020416561127 1101 14 0.8005999543639604 1101 15 0.7201219111645969 1101 16 0.8220982637070833 1101 17 0.7988612401271382 1101 18 0.7926257998287838 1101 19 0.8662874682746349 1101 20 0.7824389507695741 1101 21 0.7674622738873751 1101 22 0.8663949182140129 1101 23 0.8380856993532504 1101 24 0.8600617913842044 1101 25 0.8662930406162488 1102 1 0.5178079148915913 1102 2 0.4864513745246377 1102 3 0.4418320980126005 1102 4 0.44247592494735954 1102 5 0.47745826632013433 1102 6 0.45623967043953684 1102 7 0.4978727545151216 1102 8 0.46034227763018265 1102 9 0.4126171347783867 1102 10 0.5812104708718238 1102 11 0.5555460931007659 1102 12 0.5315426092883868 1102 13 0.6209772624499692 1102 14 0.73829372877087 1102 15 0.7865428042648333 1102 16 0.7542920302903724 1102 17 0.8758219521216475 1102 18 0.9404556941204447 1102 19 0.8192373099021282 1102 20 0.9154447536634223 1102 21 0.8672838952552553 1102 22 0.8164388422756801 1102 23 0.82530720627269 1102 24 0.8767878617118754 1102 25 0.8407986434488492 1103 1 0.2945568860850045 1103 2 0.35064890538883375 1103 3 0.30345761241589386 1103 4 0.25523312659344555 1103 5 0.4183018877193092 1103 6 0.44800921776463554 1103 7 0.5786340713875475 1103 8 0.40530112536681173 1103 9 0.5160121965067788 1103 10 0.6660870140816968 1103 11 0.5838382741707235 1103 12 0.5665220331567014 1103 13 0.64258220072157 1103 14 0.8180262390589377 1103 15 0.7006374544705949 1103 16 0.7398887381093004 1103 17 0.6402252443027194 1103 18 0.8159700974126309 1103 19 0.8367270218362819 1103 20 0.7117410499597199 1103 21 0.564814759452787 1103 22 0.654401805238642 1103 23 0.6432084408405885 1103 24 0.7929401250863606 1103 25 0.7569509068233344 1104 1 0.3350357394752024 1104 2 0.3471566472992116 1104 3 0.36656073683338164 1104 4 0.26760799524009116 1104 5 0.11098778472189252 1104 6 0.27979422023295797 1104 7 0.2762627464649299 1104 8 0.23389088707697425 1104 9 0.2617185037210277 1104 10 0.3783829682959663 1104 11 0.3245700531816311 1104 12 0.2778104328887707 1104 13 0.3282275067211202 1104 14 0.3739097939262308 1104 15 0.35532433675135877 1104 16 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0.5464019816852215 1107 15 0.5731007275695461 1107 16 0.5090744096206876 1107 17 0.5114855813102892 1107 18 0.5279106934919326 1107 19 0.521996180718638 1107 20 0.6230341727715043 1107 21 0.6134803504318498 1107 22 0.6672180975783846 1107 23 0.6809798613458327 1107 24 0.5968388744449697 1107 25 0.5832688302104714 1108 1 0.26715438950006387 1108 2 0.28246755021627834 1108 3 0.2854245469261052 1108 4 0.24916804640121626 1108 5 0.34686925712681926 1108 6 0.3733998318747851 1108 7 0.28281974336605487 1108 8 0.34332236413786177 1108 9 0.35568751394105647 1108 10 0.40597506234071545 1108 11 0.52107919611867 1108 12 0.42618884779138 1108 13 0.49241155500202893 1108 14 0.46536458400454705 1108 15 0.477912087391978 1108 16 0.67042847824316 1108 17 0.7327098095233779 1108 18 0.7400890443950416 1108 19 0.7353469520554299 1108 20 0.6927226032807425 1108 21 0.7532616743197167 1108 22 0.7823000482793248 1108 23 0.7287213537303217 1108 24 0.7332623736854913 1108 25 0.7466475616740027 1109 1 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-1.8068641362954594 2314 19 -1.5138661980444255 2314 20 -1.729287783662926 2314 21 -1.665486501071861 2314 22 -1.4976621619260655 2314 23 -1.294604189281138 2314 24 -1.5773126287625345 2314 25 -1.5613890238505093 2315 1 -0.7405703902265692 2315 2 -0.6412592536018273 2315 3 -0.7518053185123748 2315 4 -0.7166564276314347 2315 5 -0.7589485496774984 2315 6 -0.7975890917890922 2315 7 -0.883540345211351 2315 8 -0.9464706397449447 2315 9 -1.0223154691495608 2315 10 -0.9832285523163691 2315 11 -1.0977768905615406 2315 12 -1.2336184266643997 2315 13 -1.1351493731676854 2315 14 -1.3581122247541728 2315 15 -1.3483725502114012 2315 16 -1.3347089800346552 2315 17 -1.3995686247492998 2315 18 -1.296166740023608 2315 19 -1.2627160672940931 2315 20 -1.2212971945389413 2315 21 -1.2401309047743112 2315 22 -1.1572988674899705 2315 23 -1.265692892295604 2315 24 -1.1387705276821762 2315 25 -1.2768549382765855 2316 1 -1.3361088668095533 2316 2 -1.2074847638614337 2316 3 -1.1436478681030415 2316 4 -0.9884013613120786 2316 5 -1.194428242208673 2316 6 -4.603196367951899 2316 7 -2.390738710235553 2316 8 -1.7935268237025923 2316 9 -1.8148100927037163 2316 10 -1.4825832605214515 2316 11 -1.6078622118194588 2316 12 -1.6789977194419379 2316 13 -1.6996671176076887 2316 14 -1.9189823848685563 2316 15 -1.8563019699180145 2316 16 -1.886476839022329 2316 17 -1.9668631151836777 2316 18 -2.0298546388210355 2316 19 -2.10987299800005 2316 20 -2.125534551512075 2316 21 -2.288485795151601 2316 22 -2.2101475360162155 2316 23 -2.3329950718164554 2316 24 -2.351126534751401 2316 25 -2.4741003131925012 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 17715.0 3 2.0 4 395.0 5 2961.0 6 3812.0 7 2987.0 8 2325.0 9 2097.0 10 2004.0 11 1763.0 12 1796.0 13 1851.0 14 1820.0 15 1823.0 16 1843.0 17 1946.0 18 2110.0 19 2548.0 20 3085.0 21 3938.0 22 5016.0 23 6371.0 24 7751.0 25 9406.0 26 10826.0 27 12162.0 28 14290.0 29 17406.0 30 22375.0 31 28885.0 32 37604.0 33 49919.0 34 66963.0 35 90743.0 36 136548.0 37 245662.0 38 1078789.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.714233139056056 19.12826201807232 13.058142931411018 25.099361911460598 2 15.482321969198368 20.808319073318415 39.82819534026589 23.88116361721732 3 18.49519119659159 25.282213507817957 30.307964519775084 25.91463077581537 4 12.766501831299346 17.389736405557127 37.852486297500725 31.991275465642804 5 13.178158539040325 37.92307343689562 35.58628205493038 13.312485969133675 6 31.66889178549328 37.23026688259536 17.90449105344235 13.196350278469012 7 27.624676546075534 31.82202109072297 22.629926583726252 17.923375779475247 8 25.436157109765045 36.28828206414963 20.656609246093662 17.61895157999167 9 26.754595906219343 14.61464212989073 20.134942789123063 38.495819174766865 10 15.456211394358114 28.51838589580887 34.35205712450171 21.673345585331305 11 34.17163572710129 22.137039164955606 24.744525432127894 18.946799675815214 12 23.401221116446315 24.709861089315073 31.629538505612143 20.25937928862647 13 29.235253790347976 21.796883474114427 25.78684538371053 23.181017351827066 14 21.212550385605198 21.92310851279805 27.676175874719377 29.188165226877377 15 23.234630621619132 30.490688216480873 23.567617367956935 22.707063793943064 16 22.293428882340894 27.657794584725604 27.62804958622864 22.42072694670486 17 21.90009202382933 28.266530069891783 27.71543999814689 22.117937908132003 18 21.78889446934592 26.759028495077093 29.793059806705603 21.659017228871388 19 23.48251414709185 26.45033685056175 28.056385156780156 22.010763845566245 20 23.850508755822474 26.57833281390824 28.278168984425673 21.292989445843617 21 23.217183976937537 26.103203043689067 27.71470100345505 22.96491197591834 22 23.540566279604175 27.250659508370795 27.5719510339712 21.636823178053834 23 22.450576114074114 27.21526350894981 28.08415945569894 22.25000092127713 24 23.18475502188165 26.367741191669335 28.537743671220934 21.90976011522808 25 22.763599761415545 26.76457473584352 28.261360531540053 22.210464971200878 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 449.0 1 449.0 2 656.0 3 863.0 4 863.0 5 863.0 6 2021.5 7 3180.0 8 3180.0 9 3180.0 10 4165.5 11 5151.0 12 5151.0 13 5151.0 14 7348.0 15 9545.0 16 9545.0 17 9545.0 18 16646.5 19 23748.0 20 23748.0 21 23748.0 22 36485.5 23 49223.0 24 49223.0 25 49223.0 26 70185.0 27 91147.0 28 91147.0 29 91147.0 30 114917.0 31 138687.0 32 138687.0 33 138687.0 34 155838.0 35 172989.0 36 172989.0 37 172989.0 38 185684.0 39 198379.0 40 198379.0 41 198379.0 42 210798.5 43 223218.0 44 223218.0 45 223218.0 46 234128.0 47 245038.0 48 245038.0 49 245038.0 50 239581.0 51 234124.0 52 234124.0 53 234124.0 54 211010.0 55 187896.0 56 187896.0 57 187896.0 58 162398.5 59 136901.0 60 136901.0 61 136901.0 62 113643.5 63 90386.0 64 90386.0 65 90386.0 66 70373.5 67 50361.0 68 50361.0 69 50361.0 70 36805.5 71 23250.0 72 23250.0 73 23250.0 74 16530.5 75 9811.0 76 9811.0 77 9811.0 78 6700.5 79 3590.0 80 3590.0 81 3590.0 82 2385.5 83 1181.0 84 1181.0 85 1181.0 86 743.5 87 306.0 88 306.0 89 306.0 90 201.0 91 96.0 92 96.0 93 96.0 94 56.5 95 17.0 96 17.0 97 17.0 98 9.0 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.15451133618350155 2 0.015108944969221445 3 0.0 4 0.00347453089884535 5 0.10186692862523869 6 0.04011503855939631 7 0.02648013700180623 8 0.026743359039597545 9 0.026848647854714067 10 0.027743602783204535 11 0.032639532686122984 12 0.03258688827856473 13 0.01953107520411553 14 0.009739215398278633 15 0.0032639532686122986 16 0.0030533756383792476 17 0.0015266878191896238 18 0.002684864785471407 19 0.0015793322267478862 20 5.264440755826288E-5 21 0.0 22 8.423105209322061E-4 23 0.0 24 0.0 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1899537.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 69.18312930488796 #Duplication Level Percentage of deduplicated Percentage of total 1 82.08040690065637 56.785794040059265 2 12.114042592488454 16.761747501620977 3 3.019769714307682 6.267513558477988 4 1.1128462372373393 3.0796074050899547 5 0.5249228055069466 1.8157901164235817 6 0.29066150750583825 1.206532359463804 7 0.19061317725334428 0.9231051262393551 8 0.1255142842157459 0.6946776763606726 9 0.09132321125976278 0.5686222979809592 >10 0.4060764825912259 4.821215170002657 >50 0.022747061157252824 1.0836358877075933 >100 0.017120627822417283 2.28463393562034 >500 0.0024340910754952413 1.0925875178064073 >1k 0.0012931108838568392 1.6093589957400727 >5k 2.2819603832767752E-4 1.0051784114064197 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 8033 0.4228925259155258 No Hit GGTATCAACGCAGAGTACTTTTTTT 5587 0.29412430502801473 No Hit TATCAACGCAGAGTACTTTTTTTTT 5481 0.28854399782683887 No Hit GTCCTAAAGTGTGTATTTCTCATTT 3025 0.15924933286374524 No Hit CTGTAGGACGTGGAATATGGCAAGA 2964 0.15603802400269118 No Hit GTCCTACAGTGGACATTTCTAAATT 2954 0.15551157992710854 No Hit CTTTAGGACGTGAAATATGGCGAGG 2726 0.14350865500382462 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 2593 0.13650694879857567 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 2150 0.1131854762502652 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 5.264440755826288E-5 0.0 10 0.0 0.0 0.0 5.264440755826288E-5 0.0 11 0.0 0.0 0.0 5.264440755826288E-5 0.0 12 0.0 0.0 0.0 5.264440755826288E-5 0.0 13 0.0 0.0 0.0 5.264440755826288E-5 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTGCGC 75 1.4824096E-5 12.663823 10 TAGGACC 435 0.0 11.8004 4 GGACCGT 60 0.0058816858 11.082013 6 ACCCGTG 60 0.0058851456 11.081138 7 GGTATCA 1600 0.0 10.948205 1 TAGGACA 490 0.0 10.86386 4 TAGGACT 305 0.0 10.596715 4 GGCGAGG 855 0.0 10.216699 19 GTATCAA 3185 0.0 10.1628275 1 TGGCGAG 1790 0.0 10.078616 18 GTGCTAG 200 2.3646862E-11 9.996187 1 CGAAATC 200 2.5465852E-11 9.97171 13 GTCCTAA 950 0.0 9.820816 1 TGTAGGA 1775 0.0 9.748412 2 GTAGGAC 1765 0.0 9.748237 3 GTATAAG 215 9.094947E-12 9.741578 1 ACGAAAT 205 4.1836756E-11 9.72901 12 ACACCGA 80 0.0045206696 9.498868 6 AAGTCGT 180 4.1945896E-9 9.498117 7 GTCCTAC 1510 0.0 9.457131 1 >>END_MODULE