FastQCFastQC Report
Thu 26 May 2016
SRR1512660_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512660_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3143110
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT60700.19312082618807486No Hit
GTACATGGGAAGCAGTGGTATCAAC59660.18981200148897112No Hit
CCCATGTACTCTGCGTTGATACCAC40710.1295213975966479No Hit
GGTATCAACGCAGAGTACTTTTTTT36940.11752690806239681No Hit
TATCAACGCAGAGTACTTTTTTTTT35780.11383629589801184No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAACGT400.005327893414.2308934
GTCTACG1104.0783925E-610.71141051
GAGATCG1002.513971E-510.4063469
TTGTACT4750.010.3860564
GTCTTAG3850.010.2013431
TTAGACT2053.6379788E-1210.1814534
GTCTTAT2950.09.9852121
GTCCTAC7700.09.9463081
GTATAGG3700.09.8187921
TGGACCG1752.6375346E-99.7583275
TCTTATA2900.09.4872632
TAGGACG10600.09.3977594
TAGGACC4450.09.3806654
CTTATAC2850.09.3208193
GTAGGAC10950.09.27065853
TATACTG4100.09.2558665
CTTAGAC2054.3473847E-109.2558663
GTGCGAT1554.373942E-79.177402512
CGTGAAA5850.09.0560289
TTAGGAC6600.08.9122763
Produced by FastQC (version 0.11.5)