FastQCFastQC Report
Thu 26 May 2016
SRR1512660_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512660_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3143110
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT79510.25296601137090335No Hit
GTACATGGGAAGCAGTGGTATCAAC64240.20438355641387035No Hit
TATCAACGCAGAGTACTTTTTTTTT52310.16642751924049748No Hit
GGTATCAACGCAGAGTACTTTTTTT50980.16219604150029746No Hit
CCCATGTACTCTGCGTTGATACCAC47570.15134691436188996No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAATT400.00529382414.24466614
AGGACCG2050.012.05577855
GACCGTA752.07864E-411.3979087
ACTACGC752.07864E-411.3979088
ACCGTGC3150.010.5536198
GTTCTAG2001.8189894E-1210.4684051
TAGGACC4950.010.1774644
GTACCGT1606.190021E-910.0928626
ACCGTAT1054.1161242E-59.9505568
TAGGACG9200.09.7120064
GTATAGG2650.09.6962941
GTCTTAT2750.09.6897641
TCTTGCG800.0044901289.5070312
TTAGGAC6400.09.5053663
GTACCGA2106.730261E-119.4991656
GCGAACC1901.0568328E-99.49825811
ACCGCTT1401.0621279E-69.4982588
ACCGATC2201.6370905E-119.4982588
CTAAGCT1951.6680133E-99.261644
GGTTATA1856.506525E-99.2595231
Produced by FastQC (version 0.11.5)