FastQCFastQC Report
Thu 26 May 2016
SRR1512659_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512659_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2166246
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT66180.3055054689079634No Hit
GTCCTACAGTGGACATTTCTAAATT54570.2519104478438737No Hit
GTCCTAAAGTGTGTATTTCTCATTT47900.22111985434710557No Hit
CTGTAGGACGTGGAATATGGCAAGA46970.2168267131249175No Hit
GGTATCAACGCAGAGTACTTTTTTT39860.18400495603915712No Hit
CTTTAGGACGTGAAATATGGCGAGG38830.17925018672856177No Hit
TATCAACGCAGAGTACTTTTTTTTT36340.16775564732721954No Hit
GTCCTACAGTGTGCATTTCTCATTT28560.13184098204913017No Hit
GTACATGGGAAGCAGTGGTATCAAC28040.12944051598941209No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC8900.012.6848754
TATGTCG1600.012.45690416
GTCGAGG1251.4606485E-912.13407119
TGTCGAG1850.011.78097918
GTCCTAA15300.011.6153881
CTAGGAC2900.011.4498363
GGCGAGG13150.011.31801919
GAAATGT7050.011.0345336
ACCGACC600.00607698911.0335858
AGGACCT18400.010.6213245
ATCCCGT1252.3042958E-710.61854410
TCCAACG1355.9051672E-810.52889418
GTCCTAC26750.010.4975721
ATGTCCA7400.010.4819599
AGGACGT30950.010.3606095
GGACCTG17950.010.3590726
TGTAGGA30550.010.3099392
GACGTGG14100.010.2271287
AATGTCC7400.010.2241728
ATGTCGA1951.4551915E-1110.22176317
Produced by FastQC (version 0.11.5)