FastQCFastQC Report
Thu 26 May 2016
SRR1512659_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512659_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2166246
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT78670.3631628171500374No Hit
CTGTAGGACGTGGAATATGGCAAGA56350.26012742781752396No Hit
GTCCTACAGTGGACATTTCTAAATT55600.2566652171544691No Hit
GGTATCAACGCAGAGTACTTTTTTT52070.24036974563369073No Hit
TATCAACGCAGAGTACTTTTTTTTT50490.2330760218368551No Hit
GTCCTAAAGTGTGTATTTCTCATTT50170.23159881195395168No Hit
CTTTAGGACGTGAAATATGGCGAGG45530.21017926865185213No Hit
GTACATGGGAAGCAGTGGTATCAAC32370.14942901221744898No Hit
GTCCTACAGTGTGCATTTCTCATTT31510.14545901065714603No Hit
CTGAAGGACCTGGAATATGGCGAGA25200.11633027827864426No Hit
ACGCAGAGTACTTTTTTTTTTTTTT24930.1150838824399445No Hit
CTGTAGGACCTGGAATATGGCGAGA23810.10991364784978253No Hit
CCCATGTACTCTGCGTTGATACCAC22380.10331236618555788No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC9650.013.4962344
GACGTAA500.001501061313.298617
GCGAACC1501.4551915E-1112.03179611
GGACGTA1201.002627E-811.8745816
CGAACCG1604.5474735E-1111.2795512
AAATGTC6600.011.2260997
GGCGAAC1553.1468517E-1011.03084910
TGTAGGA29950.010.9837052
AATGCCG1658.185452E-1110.93244318
CTGTAGG30450.010.6607841
GGCGAGG11750.010.58427119
ATTTAGA7250.010.5044691
CCTCGAA1451.4584657E-810.47609817
GTCCTAA14350.010.4152531
GTAGGAC30100.010.3907953
TCGAACT1659.404175E-1010.35657319
AGGACCT19300.010.3440685
GTCCTAC28850.010.262111
GTATTAA2600.010.2519571
TAGAAAT7800.010.2377394
Produced by FastQC (version 0.11.5)