FastQCFastQC Report
Thu 26 May 2016
SRR1512658_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512658_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2295850
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT59770.26033930788161247No Hit
GTCCTACAGTGGACATTTCTAAATT55340.2410436221878607No Hit
CTGTAGGACGTGGAATATGGCAAGA50030.21791493346690768No Hit
GTCCTAAAGTGTGTATTTCTCATTT46130.20092776095999304No Hit
CTTTAGGACGTGAAATATGGCGAGG35920.15645621447394212No Hit
TATCAACGCAGAGTACTTTTTTTTT34130.14865953786179412No Hit
GGTATCAACGCAGAGTACTTTTTTT33880.1475706165472483No Hit
GTACATGGGAAGCAGTGGTATCAAC29860.13006076180935167No Hit
GTCCTACAGTGTGCATTTCTCATTT29520.12857982882156935No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC9850.013.7727724
CCGCCCG701.1350402E-412.1630059
GTGCGTT658.1452157E-411.67176411
CCAACGA1552.7284841E-1111.61707219
ACCGCCC752.159912E-411.3506518
AGGACCT18400.011.2914015
GGTCGCT600.005954514311.0638611
GTCTAGG1707.4578566E-1110.9779651
TCCAACG2250.010.94818418
ACCGTGC1303.4839104E-810.9140878
GTATTAG1801.8189894E-1110.9137661
GTCCTAG3600.010.9137661
GTCCTAC27150.010.88964751
GGCGAGG12050.010.85342519
CTTAGAC2100.010.8418653
AATGTCC7400.010.73710258
GGACCTG18950.010.713376
TGTAGGA32000.010.5242322
GTCCTAA14400.010.4362891
TCCTACA30250.010.3176912
Produced by FastQC (version 0.11.5)