FastQCFastQC Report
Thu 26 May 2016
SRR1512658_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512658_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2295850
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT77160.3360846745214191No Hit
CTGTAGGACGTGGAATATGGCAAGA58250.25371866628917394No Hit
GTCCTACAGTGGACATTTCTAAATT56670.24683668358124441No Hit
GGTATCAACGCAGAGTACTTTTTTT51090.2225319598405819No Hit
TATCAACGCAGAGTACTTTTTTTTT50290.21904741163403532No Hit
GTCCTAAAGTGTGTATTTCTCATTT47330.20615458326981292No Hit
CTTTAGGACGTGAAATATGGCGAGG43120.18781714833286145No Hit
GTACATGGGAAGCAGTGGTATCAAC32820.14295359017357406No Hit
GTCCTACAGTGTGCATTTCTCATTT29920.13032210292484264No Hit
ACGCAGAGTACTTTTTTTTTTTTTT26410.11503364766861947No Hit
CTGAAGGACCTGGAATATGGCGAGA26220.11420606746956465No Hit
CTGTAGGACCTGGAATATGGCGAGA25840.11255090707145501No Hit
CCCATGTACTCTGCGTTGATACCAC24550.10693207308839862No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGACC400.005284982314.2480228
AATCGCA400.005284982314.2480227
TAGGACC10300.013.4738394
CGAAATC1405.2750693E-1112.21046413
ACGAAAT1251.4169927E-912.15698912
AGGACCG1459.640644E-1111.8007235
CGCCCTA1701.8189894E-1211.72983316
ATTACAC2100.011.3160913
GTCCCGC1850.011.294512
GGCGAGG11950.011.2834519
TGTCGAG1553.1650416E-1011.02712318
TGTAGGA32150.011.0000452
TCCCGCC1855.456968E-1210.78040913
CTGTAGG31300.010.6092111
AGGACCT20800.010.6035
GTAGAAC5100.010.4374293
GTCGAGG1553.6925485E-910.41450619
CACCTTT8300.010.41104214
GTAGGAC32750.010.3036893
GGACCTG20150.010.2776096
Produced by FastQC (version 0.11.5)