##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512658_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2295850 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.591452403249342 33.0 31.0 34.0 29.0 34.0 2 31.732169349042838 34.0 31.0 34.0 29.0 34.0 3 31.832802665679377 34.0 31.0 34.0 28.0 34.0 4 35.28438791732909 37.0 35.0 37.0 33.0 37.0 5 35.05202735370342 37.0 35.0 37.0 32.0 37.0 6 34.91096848661716 37.0 35.0 37.0 32.0 37.0 7 34.89930788161247 37.0 35.0 37.0 32.0 37.0 8 34.88742992791341 37.0 35.0 37.0 32.0 37.0 9 36.47652895441775 39.0 37.0 39.0 32.0 39.0 10 36.27902127752249 39.0 37.0 39.0 32.0 39.0 11 36.390657490689726 39.0 37.0 39.0 32.0 39.0 12 36.327381144238515 39.0 37.0 39.0 32.0 39.0 13 36.32214212600997 39.0 37.0 39.0 32.0 39.0 14 37.566368011847466 40.0 38.0 41.0 32.0 41.0 15 37.55146242132543 40.0 38.0 41.0 32.0 41.0 16 37.515359452925935 40.0 38.0 41.0 32.0 41.0 17 37.4906435524969 40.0 38.0 41.0 32.0 41.0 18 37.42209595574624 40.0 38.0 41.0 32.0 41.0 19 37.44452163686652 40.0 38.0 41.0 32.0 41.0 20 37.40007143323823 40.0 38.0 41.0 32.0 41.0 21 37.35304919746499 40.0 38.0 41.0 32.0 41.0 22 37.2546773526145 40.0 38.0 41.0 32.0 41.0 23 37.201498355728816 40.0 38.0 41.0 32.0 41.0 24 37.158323496744124 40.0 38.0 41.0 32.0 41.0 25 37.08598993836706 40.0 38.0 41.0 31.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.6249684585667445 1101 2 0.5863161930108376 1101 3 0.6307565743763774 1101 4 0.5544273636131436 1101 5 0.5637526533056132 1101 6 0.8034951622764765 1101 7 0.7041200910218777 1101 8 0.7545018037637448 1101 9 0.8350260401888505 1101 10 0.9649805563814198 1101 11 0.9512781071720653 1101 12 0.8845521572590656 1101 13 0.9107420232715384 1101 14 1.0862263815947557 1101 15 0.9976804384545872 1101 16 1.0327989602977183 1101 17 1.0778886831781875 1101 18 1.071543216111941 1101 19 1.1576662582396438 1101 20 1.1993002137656887 1101 21 1.2362244677302598 1101 22 1.3106522889780692 1101 23 1.1494836045124615 1101 24 1.0929857344316147 1101 25 1.1633289892811831 1102 1 0.48216646222421744 1102 2 0.47145993481989024 1102 3 0.4699528206982464 1102 4 0.43793547914837916 1102 5 0.4710469118181564 1102 6 0.659597008020647 1102 7 0.6466438780355901 1102 8 0.5114891090360771 1102 9 0.6477415106824651 1102 10 0.8247621258744502 1102 11 0.732937382593299 1102 12 0.6857369752881439 1102 13 0.7445310087130537 1102 14 0.8695475988195867 1102 15 0.9299485217223449 1102 16 1.0038592304072793 1102 17 0.9545626943286223 1102 18 0.9693120268299467 1102 19 1.006896654766308 1102 20 0.9002031266819941 1102 21 0.8811780488771959 1102 22 0.9038151660472025 1102 23 0.9808014456096217 1102 24 0.9546595066138153 1102 25 0.9810331876337202 1103 1 0.3721890809544597 1103 2 0.4224288723183278 1103 3 0.3613250393413949 1103 4 0.47392270009029147 1103 5 0.4390061221412367 1103 6 0.6088045204920576 1103 7 0.5966734129680162 1103 8 0.603332439693844 1103 9 0.7042579513125489 1103 10 0.8369720080132481 1103 11 0.7883773264823546 1103 12 0.6801166567015358 1103 13 0.6445003705161554 1103 14 0.7340591257629328 1103 15 0.86740210774785 1103 16 0.740974277017493 1103 17 0.7629041333399798 1103 18 0.890280490467255 1103 19 0.9519832031509665 1103 20 0.859147219388035 1103 21 0.7684294842966111 1103 22 0.7141095640319506 1103 23 0.8337012044468608 1103 24 0.8111059894247745 1103 25 0.8885420056106312 1104 1 0.384658956283662 1104 2 0.39731109040260293 1104 3 0.39158650942881934 1104 4 0.3715781384996859 1104 5 0.42521699850577477 1104 6 0.5190313417483239 1104 7 0.5349276653642789 1104 8 0.36167536178953696 1104 9 0.47308777055229 1104 10 0.6440153019304518 1104 11 0.5017966203184869 1104 12 0.5314063961958553 1104 13 0.45054728341222017 1104 14 0.5099064165255385 1104 15 0.5224662846092372 1104 16 0.5901877422605111 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-1.3690906982699715 2314 14 -1.521268243165359 2314 15 -1.4991689124073986 2314 16 -1.7097404662718034 2314 17 -1.81227186832038 2314 18 -1.6483717412730314 2314 19 -1.5149853574883352 2314 20 -1.735368974082462 2314 21 -1.4383140290160839 2314 22 -1.4361048245137056 2314 23 -1.3551904230941858 2314 24 -1.5460024051962975 2314 25 -1.5271300860830337 2315 1 -0.6982352202123003 2315 2 -0.6326201819569448 2315 3 -0.6873361818592976 2315 4 -0.6383157217201259 2315 5 -0.8163656168483229 2315 6 -0.5797064667076199 2315 7 -0.875380301604288 2315 8 -0.94810516614195 2315 9 -1.2830118162079458 2315 10 -1.0593596281905562 2315 11 -1.264445477979585 2315 12 -1.262707711523916 2315 13 -1.2872674148284204 2315 14 -1.658090147439232 2315 15 -1.6723421394338516 2315 16 -1.5563098461338214 2315 17 -1.6681782727708097 2315 18 -1.460566407223169 2315 19 -1.467229744810929 2315 20 -1.4361068287871461 2315 21 -1.3836761021428785 2315 22 -1.3540884873214765 2315 23 -1.4475022172367673 2315 24 -1.4185488837413232 2315 25 -1.5560084898629043 2316 1 -1.4829643976844444 2316 2 -1.3453703473925316 2316 3 -1.3031086766279572 2316 4 -1.167437974148001 2316 5 -1.3290940968082552 2316 6 -4.63880944812518 2316 7 -2.5452278773022456 2316 8 -1.9266354274407504 2316 9 -1.8198704179642462 2316 10 -1.7428634645076926 2316 11 -1.767245587077646 2316 12 -1.8337309977006555 2316 13 -1.8959653026183858 2316 14 -2.211481129858349 2316 15 -2.1290360209998553 2316 16 -2.1388912672702247 2316 17 -2.2294486839453143 2316 18 -2.280536718242068 2316 19 -2.3303262071720994 2316 20 -2.3977056519588587 2316 21 -2.7244588807453525 2316 22 -2.5755922073865776 2316 23 -2.4880205224609497 2316 24 -2.659420863424046 2316 25 -2.64483286880877 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 24358.0 3 1.0 4 497.0 5 4119.0 6 5135.0 7 3995.0 8 3200.0 9 2850.0 10 2692.0 11 2550.0 12 2336.0 13 2334.0 14 2291.0 15 2372.0 16 2395.0 17 2556.0 18 2727.0 19 3223.0 20 3959.0 21 5110.0 22 6660.0 23 8148.0 24 9941.0 25 11755.0 26 13504.0 27 15328.0 28 17780.0 29 21850.0 30 27634.0 31 35525.0 32 46539.0 33 61356.0 34 82693.0 35 112486.0 36 167754.0 37 307992.0 38 1270205.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.39143711676247 19.271983918419483 13.230397956299141 25.106181008518906 2 15.12735440543258 20.290666369271143 40.86671917556385 23.715260049732432 3 19.148463532025175 24.56258030794695 29.822505825729035 26.46645033429884 4 12.716015719262543 17.02979638310606 37.745047221382514 32.509140676248876 5 13.010023339395268 38.148117304768554 35.73600192191396 13.105857433922216 6 32.32059545954256 36.976610121191285 17.552322103106494 13.150472316159668 7 28.299956649885743 31.47309787758712 21.87102578569359 18.35591968683355 8 25.206937250273498 37.46192698782404 20.138014805287938 17.193120956614518 9 26.549651498872894 14.725577691048239 20.012913580956912 38.711857229121954 10 15.334679306147239 28.84767706411786 34.2828337926894 21.534809837045504 11 34.44609433480516 21.85523780642429 24.865082249067797 18.833585609702748 12 23.8369298914083 24.43790300338415 31.66674727278986 20.05841983241769 13 29.51308609704297 21.72307357185645 25.625442465828023 23.13839786527256 14 20.742796829267547 22.55237253884679 27.385487412774147 29.319343219111516 15 22.982237891510792 31.496707445728827 23.1403972321462 22.38065743061418 16 21.747450007426632 28.006077208247614 28.374229187238736 21.872243597087017 17 21.65541507337456 28.861589068495263 27.872183526343274 21.610812331786903 18 21.496919603033007 26.919411379348794 30.581564311998218 21.00210470561998 19 23.67304039365267 26.50125379437772 27.85845299588689 21.967252816082716 20 23.939325304353506 26.894657752030838 28.37998998192391 20.786026961691746 21 22.737983753293985 26.38038199359714 27.540083193588433 23.341551059520437 22 23.338367099640262 28.139552205325398 27.26156869697177 21.260511998062572 23 21.892675915238367 27.83714092819653 28.26979114489187 22.000392011673238 24 23.191986929453982 26.651883464461296 28.67409834009118 21.48203126599354 25 22.40747435590304 27.28479648060631 28.384389224034674 21.923339939455975 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 189.0 1 189.0 2 378.0 3 567.0 4 567.0 5 567.0 6 1525.0 7 2483.0 8 2483.0 9 2483.0 10 3579.0 11 4675.0 12 4675.0 13 4675.0 14 6969.5 15 9264.0 16 9264.0 17 9264.0 18 17112.0 19 24960.0 20 24960.0 21 24960.0 22 40730.0 23 56500.0 24 56500.0 25 56500.0 26 82887.5 27 109275.0 28 109275.0 29 109275.0 30 142065.0 31 174855.0 32 174855.0 33 174855.0 34 196877.0 35 218899.0 36 218899.0 37 218899.0 38 233978.5 39 249058.0 40 249058.0 41 249058.0 42 263232.5 43 277407.0 44 277407.0 45 277407.0 46 293575.5 47 309744.0 48 309744.0 49 309744.0 50 296378.0 51 283012.0 52 283012.0 53 283012.0 54 250180.0 55 217348.0 56 217348.0 57 217348.0 58 187252.5 59 157157.0 60 157157.0 61 157157.0 62 129675.0 63 102193.0 64 102193.0 65 102193.0 66 79435.5 67 56678.0 68 56678.0 69 56678.0 70 41374.5 71 26071.0 72 26071.0 73 26071.0 74 18139.0 75 10207.0 76 10207.0 77 10207.0 78 6968.0 79 3729.0 80 3729.0 81 3729.0 82 2456.0 83 1183.0 84 1183.0 85 1183.0 86 747.5 87 312.0 88 312.0 89 312.0 90 191.5 91 71.0 92 71.0 93 71.0 94 40.0 95 9.0 96 9.0 97 9.0 98 6.5 99 4.0 100 4.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.15410414443452317 2 0.01594180804495067 3 0.0 4 0.002961865975564606 5 0.1003114314959601 6 0.03846070082975804 7 0.025480758760371976 8 0.025654986170699303 9 0.0257856567284448 10 0.026482566369754122 11 0.03258052573121066 12 0.03218851405797417 13 0.02068950497637041 14 0.009974519241239628 15 0.002787638565237276 16 0.002569854302328114 17 0.0011760350197094758 18 0.0018293878084369621 19 8.275801990548163E-4 20 0.0 21 0.0 22 9.146939042184811E-4 23 0.0 24 4.355685258183244E-5 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2295850.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.66051978790982 #Duplication Level Percentage of deduplicated Percentage of total 1 78.660749032893 50.075841703402936 2 13.82429435550171 17.601235287446134 3 3.9478308154810753 7.539628852447596 4 1.4822595099631903 3.7744564345931675 5 0.7167028300734924 2.281283734797227 6 0.3746598962061579 1.4310626241701012 7 0.2315990424888338 1.0320600799054962 8 0.14448509626015657 0.7358397063622202 9 0.10354991417264947 0.5932837224201885 >10 0.45235184252176586 5.050012568196913 >50 0.03137216026277083 1.3822915141173882 >100 0.024463188404076158 3.2853302029037814 >500 0.0034230817899566016 1.5783159644867057 >1k 0.0019169258023741746 2.3603699123163135 >5k 3.423081789953883E-4 1.2789876924338885 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 7716 0.3360846745214191 No Hit CTGTAGGACGTGGAATATGGCAAGA 5825 0.25371866628917394 No Hit GTCCTACAGTGGACATTTCTAAATT 5667 0.24683668358124441 No Hit GGTATCAACGCAGAGTACTTTTTTT 5109 0.2225319598405819 No Hit TATCAACGCAGAGTACTTTTTTTTT 5029 0.21904741163403532 No Hit GTCCTAAAGTGTGTATTTCTCATTT 4733 0.20615458326981292 No Hit CTTTAGGACGTGAAATATGGCGAGG 4312 0.18781714833286145 No Hit GTACATGGGAAGCAGTGGTATCAAC 3282 0.14295359017357406 No Hit GTCCTACAGTGTGCATTTCTCATTT 2992 0.13032210292484264 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 2641 0.11503364766861947 No Hit CTGAAGGACCTGGAATATGGCGAGA 2622 0.11420606746956465 No Hit CTGTAGGACCTGGAATATGGCGAGA 2584 0.11255090707145501 No Hit CCCATGTACTCTGCGTTGATACCAC 2455 0.10693207308839862 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 8.711370516366488E-5 7 0.0 0.0 0.0 4.355685258183244E-5 8.711370516366488E-5 8 0.0 0.0 0.0 4.355685258183244E-5 8.711370516366488E-5 9 0.0 0.0 0.0 8.711370516366488E-5 8.711370516366488E-5 10 0.0 0.0 0.0 8.711370516366488E-5 8.711370516366488E-5 11 0.0 0.0 0.0 8.711370516366488E-5 8.711370516366488E-5 12 0.0 0.0 0.0 8.711370516366488E-5 1.3067055774549732E-4 13 0.0 0.0 0.0 8.711370516366488E-5 2.177842629091622E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACCGACC 40 0.0052849823 14.248022 8 AATCGCA 40 0.0052849823 14.248022 7 TAGGACC 1030 0.0 13.473839 4 CGAAATC 140 5.2750693E-11 12.210464 13 ACGAAAT 125 1.4169927E-9 12.156989 12 AGGACCG 145 9.640644E-11 11.800723 5 CGCCCTA 170 1.8189894E-12 11.729833 16 ATTACAC 210 0.0 11.316091 3 GTCCCGC 185 0.0 11.2945 12 GGCGAGG 1195 0.0 11.28345 19 TGTCGAG 155 3.1650416E-10 11.027123 18 TGTAGGA 3215 0.0 11.000045 2 TCCCGCC 185 5.456968E-12 10.780409 13 CTGTAGG 3130 0.0 10.609211 1 AGGACCT 2080 0.0 10.603 5 GTAGAAC 510 0.0 10.437429 3 GTCGAGG 155 3.6925485E-9 10.414506 19 CACCTTT 830 0.0 10.411042 14 GTAGGAC 3275 0.0 10.303689 3 GGACCTG 2015 0.0 10.277609 6 >>END_MODULE