FastQCFastQC Report
Thu 26 May 2016
SRR1512657_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512657_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2555041
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT76920.3010519204975576No Hit
GTCCTACAGTGGACATTTCTAAATT67330.2635182762233561No Hit
CTGTAGGACGTGGAATATGGCAAGA60400.23639542379163386No Hit
GTCCTAAAGTGTGTATTTCTCATTT60000.23482989118374223No Hit
CTTTAGGACGTGAAATATGGCGAGG50390.1972179702791462No Hit
GGTATCAACGCAGAGTACTTTTTTT47330.18524164582877534No Hit
TATCAACGCAGAGTACTTTTTTTTT44790.1753005137686636No Hit
GTCCTACAGTGTGCATTTCTCATTT41010.16050623062408784No Hit
GTACATGGGAAGCAGTGGTATCAAC30140.11796288200463319No Hit
CTGTAGGACCTGGAATATGGCGAGA29120.11397077385450957No Hit
CTGAAGGACCTGGAATATGGCGAGA27110.1061039724998542No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC12500.012.9021384
GGCGAGG18200.012.34235219
GTTCTAG2800.011.5774171
AGGACGT38550.011.3940825
AGGACCT23850.011.3762835
GACGTGA20500.011.2916837
GGACGTG37900.011.1889946
TTAGGAC24100.011.0618843
GGACCTG23500.011.0209116
GTCCTAT3400.010.9789411
TGGCGAG40250.010.87663518
TGTAGGA40950.010.8425592
GTAGGAC40050.010.801953
TAGAAAT10900.010.79239754
TAGGACG42600.010.6226174
GTCCTAC35150.010.5917981
TGAAATA26300.010.45565411
ACGTGAA24200.010.3544578
GTGAAAT22450.010.30439810
GTATAGA3150.010.2910381