FastQCFastQC Report
Thu 26 May 2016
SRR1512657_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512657_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2555041
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT100260.3924007481680333No Hit
CTGTAGGACGTGGAATATGGCAAGA71580.2801520601822045No Hit
GTCCTACAGTGGACATTTCTAAATT71240.27882135746549663No Hit
GGTATCAACGCAGAGTACTTTTTTT66970.2621092968762537No Hit
TATCAACGCAGAGTACTTTTTTTTT65780.2574518373677761No Hit
GTCCTAAAGTGTGTATTTCTCATTT65390.2559254430750818No Hit
CTTTAGGACGTGAAATATGGCGAGG59520.23295125205427233No Hit
GTCCTACAGTGTGCATTTCTCATTT44980.17604414175741212No Hit
CTGTAGGACCTGGAATATGGCGAGA34520.13510546406104637No Hit
CTGAAGGACCTGGAATATGGCGAGA33900.13267888851881438No Hit
GTACATGGGAAGCAGTGGTATCAAC33740.13205267547565774No Hit
ACGCAGAGTACTTTTTTTTTTTTTT32130.12575140672889398No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC11650.013.7088534
GCGGGCC604.1054233E-412.66234713
CGATTTT550.003080836312.08489314
GGCGAGG16050.011.89034619
AGGACCG1354.6838977E-911.2668885
TAGACCG600.00584785611.0908425
TGTAGGA41400.011.0691812
GTAGGAC41050.010.6296093
GGACCTG23700.010.6222766
CTGTAGG41150.010.6143841
AGGACCT24200.010.6063555
ATTTCTA24050.010.57997615
GGACCGT909.520453E-510.5554696
CCAACGA1451.4577381E-810.47672319
TCCAACG2001.8189894E-1210.44398318
GTAAGAC2650.010.403273
GGTATCA27350.010.3335871
AATCCCG2300.010.32014219
GTCCTAT3050.010.2959421
GTCCTAA20600.010.2550491
Produced by FastQC (version 0.11.5)