FastQCFastQC Report
Thu 26 May 2016
SRR1512656_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512656_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2551503
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT76470.2997057028739531No Hit
GGTATCAACGCAGAGTACTTTTTTT47520.18624316726258994No Hit
TATCAACGCAGAGTACTTTTTTTTT46130.18079539784981638No Hit
GTCCTACAGTGGACATTTCTAAATT36100.14148523438929916No Hit
GTCCTAAAGTGTGTATTTCTCATTT32040.12557304459371593No Hit
CTGTAGGACGTGGAATATGGCAAGA29920.11726421642459366No Hit
CTTTAGGACGTGAAATATGGCGAGG27470.10766203292725895No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAACGC550.003099924712.07492353
TAGGACC7450.011.715114
GTCCTAA10250.011.3980841
GGTATCA18200.011.3820311
AAGGCGT1909.094947E-1210.48529156
AGGACGA1201.5523165E-610.277255
GTATCAA37400.010.2114341
GTCTTAT2500.010.2103851
AGGACCT15550.010.188285
CGCCCTA1409.8338205E-810.16872416
CTACCGT750.002679532910.1191394
TCCTAAA12850.09.9673492
TGTAGGA21200.09.8454572
GTACTAG1302.7877632E-69.81767751
GGCGAGG9950.09.81246319
CTAAAGT12950.09.743094
TTATACT2550.09.6727064
GTAGGAC20950.09.6459423
GTATAAG2750.09.6391741
GTGTTAT2251.2732926E-119.5995071
Produced by FastQC (version 0.11.5)