FastQCFastQC Report
Thu 26 May 2016
SRR1512656_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512656_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2551503
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT98790.3871835541639575No Hit
GGTATCAACGCAGAGTACTTTTTTT67810.26576492365480264No Hit
TATCAACGCAGAGTACTTTTTTTTT65140.255300503271993No Hit
GTCCTACAGTGGACATTTCTAAATT35940.14085815301804466No Hit
CTGTAGGACGTGGAATATGGCAAGA35450.1389377163185777No Hit
GTCCTAAAGTGTGTATTTCTCATTT33060.12957068833546345No Hit
CTTTAGGACGTGAAATATGGCGAGG32880.12886522179280213No Hit
ACGCAGAGTACTTTTTTTTTTTTTT30180.11828322365288223No Hit
GTACTTTTTTTTTTTTTTTTTTTTT25940.10166556731463769No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAACCGT500.001500742713.2991356
TAGGACC7950.012.6755354
GGTATCA20600.011.686881
CCAACGT951.3694302E-510.993896519
GTATTAA3300.010.9575881
GTAGGAC22950.010.4386773
GTCTAAC1553.5670382E-910.4366851
TGTAGGA23600.010.4347852
TAATACT3800.010.0070014
GACTGCG951.6471435E-49.9985667
GTAATAC2105.456968E-129.959353
GTTCTAG3350.09.9418811
GTGCGCC1151.0287184E-59.91162111
CTGTAGG23400.09.8817931
GTATCAA40100.09.8717531
AGGACGT23150.09.8559025
GACGTGG11300.09.8348727
GGACGTG22800.09.7077036
GGCGAGG9700.09.69049619
CCTACAC3650.09.636883
Produced by FastQC (version 0.11.5)