FastQCFastQC Report
Thu 26 May 2016
SRR1512655_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512655_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2274061
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT63640.27985177178624493No Hit
GGTATCAACGCAGAGTACTTTTTTT40850.17963458324117074No Hit
GTCCTACAGTGGACATTTCTAAATT36520.1605937571595485No Hit
TATCAACGCAGAGTACTTTTTTTTT35590.15650415710044716No Hit
GTCCTAAAGTGTGTATTTCTCATTT33570.1476213698753024No Hit
CTGTAGGACGTGGAATATGGCAAGA32320.14212459560231674No Hit
CTTTAGGACGTGAAATATGGCGAGG27280.11996160173363864No Hit
GTACATGGGAAGCAGTGGTATCAAC23130.10171231114732632No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATACCG400.005328789414.2302895
TAGGACC8300.012.8015464
TACACCG701.1055135E-412.1973925
ACCTCGT550.00317485512.0363788
ACCGTCC550.00317485512.0363788
AGCGTCA600.00595945111.0626210
TGCGCAC600.00595945111.0626210
GTATAAG2350.010.8702561
TCGAACT700.001522878810.83038719
ACTGCGT803.9329706E-410.63929758
GTAGGAC21850.010.5503713
GTTCTAG2900.010.5026291
AGGACCT16150.010.4561065
TGTAGGA22700.010.4478532
GTCCTAC19150.010.3124451
GACGTGG10500.010.3000197
CCGGTTT1201.5565329E-610.27493212
GCCGGTT1201.559627E-610.27334211
TCCAACG1856.366463E-1110.24269718
TGGCGAG20450.010.2389418
Produced by FastQC (version 0.11.5)