FastQCFastQC Report
Thu 26 May 2016
SRR1512655_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512655_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2274061
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT79590.34999061150954175No Hit
GGTATCAACGCAGAGTACTTTTTTT55030.24198999059391987No Hit
TATCAACGCAGAGTACTTTTTTTTT53880.23693295826277305No Hit
GTCCTACAGTGGACATTTCTAAATT39480.17361011863797848No Hit
GTCCTAAAGTGTGTATTTCTCATTT36570.16081362813046793No Hit
CTGTAGGACGTGGAATATGGCAAGA36340.15980222166423858No Hit
CTTTAGGACGTGAAATATGGCGAGG32450.14269626012670725No Hit
GTACATGGGAAGCAGTGGTATCAAC25660.11283778227584924No Hit
ACGCAGAGTACTTTTTTTTTTTTTT25470.11200227258635542No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATACCG453.5211815E-516.8994165
AACCGTG604.0991622E-412.6645257
AAAACCG550.003054906412.0984465
GGCGTCC658.0388004E-411.6900748
TAGGACC7100.011.6478444
ATACCGT855.330734E-511.1753196
GCGTGCG600.00588543911.0812169
AGGCGTG1752.0008883E-1110.8553087
GGCGAGG9400.010.80867419
GGCGTGC1252.2472523E-710.6379678
CCAACGA1451.4550096E-810.47776119
GCGACTA1106.082546E-610.35869516
CGTGCGC750.002652578510.13139710
TGGCGAG19650.010.09950718
GGTATCA20500.09.9801691
TGTAGGA21800.09.8564982
AAGGCGT1554.071626E-89.8054416
GTGCGCC1554.0772647E-89.80457911
AGGACCT15250.09.7864255
TGCGCCA1651.0264557E-89.7855812
Produced by FastQC (version 0.11.5)