FastQCFastQC Report
Thu 26 May 2016
SRR1512654_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512654_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2399086
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT97650.4070300105957019No Hit
GTCCTAAAGTGTGTATTTCTCATTT87470.3645971840942759No Hit
CTGTAGGACGTGGAATATGGCAAGA85170.35501019971772585No Hit
GTATCAACGCAGAGTACTTTTTTTT79730.33233489754014656No Hit
CTTTAGGACGTGAAATATGGCGAGG72000.3001142935267848No Hit
GTCCTACAGTGTGCATTTCTCATTT49820.207662418104228No Hit
GGTATCAACGCAGAGTACTTTTTTT48590.2025354655898121No Hit
TATCAACGCAGAGTACTTTTTTTTT44530.18561235403816287No Hit
CTGAAGGACCTGGAATATGGCGAGA39100.1629787344013512No Hit
CTGTAGGACCTGGAATATGGCGAGA34660.14447168630053278No Hit
GTCCTTCAGTGTGCATTTCTCATTT34600.14422159105592713No Hit
ATTTAGAAATGTCCACTGTAGGACG30860.12863232080884138No Hit
GAATATGGCAAGAAAACTGAAAATC29860.12446406673208048No Hit
TTTCTAAATTTTCCACCTTTTTCAG27480.11454362202938952No Hit
GGAATATGGCGAGAAAACTGAAAAT26080.10870806632192426No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC14400.012.8460754
GTGTAGG4800.012.687241
GGCGAGG21450.012.2833119
GCGTGCG550.00316592512.0409519
GTTTAGG4250.011.7868541
CCGTGCA658.3098095E-411.6439969
TCGAACT1850.011.27064719
TCCAACG1850.011.26781618
ACCGGGG855.3798787E-511.16530615
ACCTTTT10850.011.10870715
CCAACGT951.3970184E-510.97405119
GTCCTAC49600.010.7927351
GTTCTAA3200.010.7432271
AGGACCT31650.010.670255
AAATGTC10950.010.655887
GGACGTG52950.010.5702286
CTAGGAC4050.010.5403683
GTCGAGG1803.6379788E-1110.53065619
CACCTTT11100.010.51651114
TGTAGGA51400.010.482952
Produced by FastQC (version 0.11.5)