FastQCFastQC Report
Thu 26 May 2016
SRR1512654_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512654_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2399086
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT101650.42370302690274547No Hit
GTCCTACAGTGGACATTTCTAAATT101400.4226609633835553No Hit
CTGTAGGACGTGGAATATGGCAAGA101070.4212854395382241No Hit
GTCCTAAAGTGTGTATTTCTCATTT91130.37985299401522077No Hit
CTTTAGGACGTGAAATATGGCGAGG87170.3633467078712476No Hit
GGTATCAACGCAGAGTACTTTTTTT66720.27810591200148727No Hit
TATCAACGCAGAGTACTTTTTTTTT66410.2768137532376913No Hit
GTCCTACAGTGTGCATTTCTCATTT53580.223335053432849No Hit
CTGAAGGACCTGGAATATGGCGAGA47630.19853394167612168No Hit
CTGTAGGACCTGGAATATGGCGAGA43530.18144409996140196No Hit
GTCCTTCAGTGTGCATTTCTCATTT35180.14663917842044846No Hit
TTTCTAAATTTTCCACCTTTTTCAG34810.1450969244120469No Hit
ATTTAGAAATGTCCACTGTAGGACG34800.1450552418712793No Hit
GAATATGGCAAGAAAACTGAAAATC34740.14480514662667365No Hit
ACGCAGAGTACTTTTTTTTTTTTTT31890.1329256225079051No Hit
GGAATATGGCGAGAAAACTGAAAAT30650.12775698745272157No Hit
ATTCCAGGTCCTTCAGTGTGCATTT27430.1143352093255515No Hit
TTCCAGGTCCTTCAGTGTGCATTTC26360.10987517746341732No Hit
GTACATGGGAAGCAGTGGTATCAAC24900.1037895265113464No Hit
GTACTTTTTTTTTTTTTTTTTTTTT24470.10199717725833922No Hit
GCCATATTCCACGTCCTACAGTGGA24170.10074670103531094No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTTCG250.00605323518.98899517
CGAGTAA601.4697907E-615.83109310
TAGGACC14500.013.7684434
TCCAACG1750.012.47848218
CCAACGA1155.3751137E-912.38412919
GGCGAGG24650.011.94034319
CCACCTT10700.011.09355313
TACGTCC600.00584518711.0914775
TGCGATT600.00588708411.08084112
CGAAATT600.00590263311.07691419
TAGAAAT12900.011.0543994
TGTAGGA52950.010.9886972
TAGGACT5800.010.8180634
AAATGTC13100.010.8038147
CGCCCTA1855.456968E-1210.77753816
CTGTAGG51500.010.7374521
AGGACGT54050.010.71184355
TAGTACG803.7474875E-410.6951294
GTAGGAC53500.010.661593
AGTCGTC4200.010.6294488
Produced by FastQC (version 0.11.5)