FastQCFastQC Report
Thu 26 May 2016
SRR1512653_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512653_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2263106
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT84930.3752806982969423No Hit
GTCCTAAAGTGTGTATTTCTCATTT80000.3534964778494688No Hit
CTGTAGGACGTGGAATATGGCAAGA76270.33701470456973737No Hit
GTATCAACGCAGAGTACTTTTTTTT75290.33268437271608137No Hit
CTTTAGGACGTGAAATATGGCGAGG66010.291678781285543No Hit
GGTATCAACGCAGAGTACTTTTTTT47910.21170020317210062No Hit
TATCAACGCAGAGTACTTTTTTTTT43450.19199277453199276No Hit
GTCCTACAGTGTGCATTTCTCATTT39090.1727272164891967No Hit
GTACATGGGAAGCAGTGGTATCAAC38880.17179928823484186No Hit
CTGAAGGACCTGGAATATGGCGAGA35800.1581896738376373No Hit
GTCCTTCAGTGTGCATTTCTCATTT33230.14683359948672312No Hit
ATTTAGAAATGTCCACTGTAGGACG31130.13755431694317458No Hit
GAATATGGCAAGAAAACTGAAAATC27570.12182372367887319No Hit
TTTCTAAATTTTCCACCTTTTTCAG27170.12005624128962585No Hit
CCCATGTACTCTGCGTTGATACCAC24800.10958390813333534No Hit
CTGTAGGACCTGGAATATGGCGAGA24500.10825829634139983No Hit
GGAATATGGCGAGAAAACTGAAAAT23510.10388377742801265No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTAGG4200.013.5653991
TAGGACC12150.013.1948984
TCCAACG2400.012.63347918
CCAACGA1950.012.15081419
TGTACGA658.123809E-411.6754122
TGTTCGA658.152636E-411.67048410
CACCTTT11100.011.37224914
GCCGGTT1451.2551027E-911.11837811
GGCGAGG20300.011.01833419
GTTCTAA2600.010.9566681
CCACCTT11050.010.905213
AGGACCT26900.010.8263425
GTCCTAC39050.010.7665511
ACCTTTT11500.010.72909815
AAATGTC11150.010.6348357
GTCCTAA23100.010.5461411
TGTAGGA46100.010.4945752
GGACCTG26850.010.4931966
AATGTCC11500.010.4431798
CCTTTTT12100.010.43262716
Produced by FastQC (version 0.11.5)