FastQCFastQC Report
Thu 26 May 2016
SRR1512653_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512653_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2263106
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT97500.43082383237904015No Hit
GTCCTACAGTGGACATTTCTAAATT90460.3997161423282869No Hit
CTGTAGGACGTGGAATATGGCAAGA89880.3971532928638782No Hit
GTCCTAAAGTGTGTATTTCTCATTT85010.3756341947747918No Hit
CTTTAGGACGTGAAATATGGCGAGG79330.3505359448474795No Hit
GGTATCAACGCAGAGTACTTTTTTT63840.28209018932387614No Hit
TATCAACGCAGAGTACTTTTTTTTT62160.2746667632890373No Hit
CTGAAGGACCTGGAATATGGCGAGA45890.20277441710640157No Hit
GTACATGGGAAGCAGTGGTATCAAC43120.19053460156086371No Hit
GTCCTACAGTGTGCATTTCTCATTT39810.17590868478984192No Hit
ATTTAGAAATGTCCACTGTAGGACG36140.15969203386849753No Hit
TTTCTAAATTTTCCACCTTTTTCAG34600.15288722666989527No Hit
GTCCTTCAGTGTGCATTTCTCATTT33690.14886620423435756No Hit
ACGCAGAGTACTTTTTTTTTTTTTT31410.1387915546156477No Hit
CTGTAGGACCTGGAATATGGCGAGA30200.1334449203881745No Hit
GAATATGGCAAGAAAACTGAAAATC29890.1320751215365078No Hit
CCCATGTACTCTGCGTTGATACCAC29670.13110300622242174No Hit
GGAATATGGCGAGAAAACTGAAAAT26490.11705152122790537No Hit
GTACTTTTTTTTTTTTTTTTTTTTT26280.1161235929735505No Hit
ATTCCAGGTCCTTCAGTGTGCATTT23060.10189535974010938No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGACGT400.005254503314.2604375
TAGGACC11600.013.3583234
TAGAGCG550.003052406512.0997645
GGCGAGG21500.011.7031219
ACCTTTT9900.011.701406515
CCAACGT1201.2854616E-711.07748219
TGTAGGA46500.010.877982
AGGACCT28300.010.8506975
GCGATAA1158.846255E-710.73678211
ATTTAGA11800.010.7259091
CCACCTT10700.010.65046413
GGACCTG28200.010.6105556
TAGAAAT12150.010.4845754
CTGTAGG47100.010.4658211
CACCTTT11000.010.44586814
AAATGTC11300.010.42272957
ATTGCGA1106.0830116E-610.35862317
TTCCACC11650.010.35395911
TGGCGAG48150.010.35324518
GTAGGAC47550.010.3362343
Produced by FastQC (version 0.11.5)