##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512653_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2263106 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.83403870609684 33.0 31.0 34.0 30.0 34.0 2 31.973733002342797 34.0 31.0 34.0 30.0 34.0 3 32.0746107340973 34.0 31.0 34.0 30.0 34.0 4 35.50133710042746 37.0 35.0 37.0 33.0 37.0 5 35.300028809962946 37.0 35.0 37.0 33.0 37.0 6 35.16340772372129 37.0 35.0 37.0 32.0 37.0 7 35.168731380677706 37.0 35.0 37.0 32.0 37.0 8 35.16877733521983 37.0 35.0 37.0 32.0 37.0 9 36.800902830004425 39.0 37.0 39.0 33.0 39.0 10 36.608346228590264 39.0 37.0 39.0 32.0 39.0 11 36.711005582593124 39.0 37.0 39.0 33.0 39.0 12 36.669517468470325 39.0 37.0 39.0 33.0 39.0 13 36.66716318192785 39.0 37.0 39.0 33.0 39.0 14 37.943714965185016 40.0 38.0 41.0 33.0 41.0 15 37.943490053050986 40.0 38.0 41.0 33.0 41.0 16 37.91323252202946 40.0 38.0 41.0 33.0 41.0 17 37.90332180640235 40.0 38.0 41.0 33.0 41.0 18 37.84631475503136 40.0 38.0 41.0 33.0 41.0 19 37.86897829796748 40.0 38.0 41.0 32.0 41.0 20 37.83488665577308 40.0 38.0 41.0 32.0 41.0 21 37.79386206390686 40.0 38.0 41.0 32.0 41.0 22 37.70285837251989 40.0 38.0 41.0 32.0 41.0 23 37.649196281570546 40.0 38.0 41.0 32.0 41.0 24 37.629182194735904 40.0 38.0 41.0 32.0 41.0 25 37.565555921817186 40.0 38.0 41.0 32.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.4547366631431409 1101 2 0.4471937953637237 1101 3 0.45299771758626406 1101 4 0.3108385170794463 1101 5 0.3628931744289048 1101 6 0.5947934039783149 1101 7 0.4582036542365273 1101 8 0.5009889445357416 1101 9 0.6258425086551966 1101 10 0.6926336540679472 1101 11 0.6330994184601195 1101 12 0.590177465266791 1101 13 0.5663631833348006 1101 14 0.7914243699670465 1101 15 0.6886076666964911 1101 16 0.6946792959256385 1101 17 0.6292581904116119 1101 18 0.7679559104667391 1101 19 0.7454956225738769 1101 20 0.795166633082971 1101 21 0.7146090046589464 1101 22 0.8166234894737556 1101 23 0.7954835468655261 1101 24 0.7568057609833119 1101 25 0.8425963393354436 1102 1 0.278996453810084 1102 2 0.29442344341200766 1102 3 0.3009935314220584 1102 4 0.3218263698314985 1102 5 0.2923510479419775 1102 6 0.42756322582613393 1102 7 0.4443548970195579 1102 8 0.4407339397130414 1102 9 0.46071597326930913 1102 10 0.6029409031144866 1102 11 0.5868401918568509 1102 12 0.4952598777861894 1102 13 0.529102884513577 1102 14 0.5812994683779351 1102 15 0.6617570760654985 1102 16 0.6229672135739932 1102 17 0.5452296824180181 1102 18 0.6768151061748853 1102 19 0.622766838222212 1102 20 0.6439308904964491 1102 21 0.6943685611276393 1102 22 0.5863266851305511 1102 23 0.7104240282081449 1102 24 0.6436842814633366 1102 25 0.6972869926512217 1103 1 0.3887840590462055 1103 2 0.3600915505295177 1103 3 0.37088944784912314 1103 4 0.29872176784929394 1103 5 0.3608357816498611 1103 6 0.5040226626921083 1103 7 0.37611769895469394 1103 8 0.35576646941635914 1103 9 0.3404540139883494 1103 10 0.5526506130539772 1103 11 0.4558199968507992 1103 12 0.47961826501686744 1103 13 0.5589994130844289 1103 14 0.6060046350627815 1103 15 0.5967479799914415 1103 16 0.5856687188738192 1103 17 0.5927383528608487 1103 18 0.7064260314110911 1103 19 0.6382255730357898 1103 20 0.5709866893153972 1103 21 0.53607104777047 1103 22 0.5704593443405344 1103 23 0.5667202139784777 1103 24 0.640261823820822 1103 25 0.6607379914977187 1104 1 0.2672095692110297 1104 2 0.2552497707829602 1104 3 0.2837683731275433 1104 4 0.1804701506556583 1104 5 0.31116929635070534 1104 6 0.4297253701976089 1104 7 0.30367731665396747 1104 8 0.2415507373419743 1104 9 0.31191024854233973 1104 10 0.5107298028724827 1104 11 0.4249952143544107 1104 12 0.40609975362277595 1104 13 0.36806613984053627 1104 14 0.3533877217179793 1104 15 0.47324714734968865 1104 16 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0.5199810283501165 1107 16 0.4782637285542606 1107 17 0.4568010907929292 1107 18 0.6194195705352854 1107 19 0.5463092816220083 1107 20 0.6123221545202071 1107 21 0.7413017467197633 1107 22 0.6897810611496951 1107 23 0.6644719597201743 1107 24 0.6273683521333808 1107 25 0.6588900069864181 1108 1 0.23677643364408496 1108 2 0.31387843642649926 1108 3 0.2768070776199707 1108 4 0.17596073683138513 1108 5 0.3071879004790432 1108 6 0.3314899965814604 1108 7 0.2590995489090204 1108 8 0.26473823159913223 1108 9 0.32277658438431445 1108 10 0.44753126756766193 1108 11 0.4351519798525061 1108 12 0.31738386333292823 1108 13 0.33812090321509913 1108 14 0.47819574670342746 1108 15 0.4984557523414139 1108 16 0.5621848632798745 1108 17 0.5461161677065505 1108 18 0.42044962249890006 1108 19 0.4864204973775017 1108 20 0.4710780352783033 1108 21 0.515359343572527 1108 22 0.638416769178697 1108 23 0.5546387174933116 1108 24 0.5937270130658234 1108 25 0.6920061117566618 1109 1 0.09410294398292507 1109 2 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17 -1.6511928333238615 2314 18 -1.5683611532352515 2314 19 -1.5259534404390038 2314 20 -1.6710961105138793 2314 21 -1.597107979774755 2314 22 -1.4024986835470656 2314 23 -1.3663579754377366 2314 24 -1.5116093097404573 2314 25 -1.6056618058879977 2315 1 -0.8425629723008257 2315 2 -0.8025602121222413 2315 3 -0.8553716784119985 2315 4 -0.6087329396054102 2315 5 -0.7692107483966666 2315 6 -0.7361893775199277 2315 7 -0.9924758696388736 2315 8 -1.1087509951011043 2315 9 -1.2011350276006922 2315 10 -1.162036501633132 2315 11 -1.2203632793324743 2315 12 -1.4114483260497224 2315 13 -1.1511849970999748 2315 14 -1.3802010511429046 2315 15 -1.505476822233362 2315 16 -1.438612405407305 2315 17 -1.5180976579751615 2315 18 -1.3258480008629405 2315 19 -1.2859448511346372 2315 20 -1.383539865211155 2315 21 -1.2597142480148236 2315 22 -1.2716093977282057 2315 23 -1.3994775522192882 2315 24 -1.3472002636412697 2315 25 -1.4726232418635163 2316 1 -1.2259576095416165 2316 2 -1.193972334303595 2316 3 -1.1919212457307964 2316 4 -1.0235755929096015 2316 5 -1.1894037224357916 2316 6 -4.931956742518064 2316 7 -2.3668845978937583 2316 8 -1.716708064596638 2316 9 -1.718429837203189 2316 10 -1.5399653180234907 2316 11 -1.4388255285639602 2316 12 -1.6446181822964974 2316 13 -1.6541506846810492 2316 14 -1.8981604306407505 2316 15 -1.9316740253536366 2316 16 -1.948138499548783 2316 17 -1.9628530642690336 2316 18 -2.1611235130615327 2316 19 -2.0735916695506873 2316 20 -2.1891531708244614 2316 21 -2.3473788356794074 2316 22 -2.2771178647751356 2316 23 -2.3600333743135735 2316 24 -2.179197349484504 2316 25 -2.1400859708647104 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 17616.0 3 0.0 4 350.0 5 3051.0 6 4037.0 7 3134.0 8 2413.0 9 2217.0 10 2109.0 11 2042.0 12 1899.0 13 1945.0 14 1849.0 15 1878.0 16 1926.0 17 2007.0 18 2401.0 19 2681.0 20 3305.0 21 4396.0 22 5618.0 23 7118.0 24 8620.0 25 10354.0 26 11987.0 27 13668.0 28 15805.0 29 19642.0 30 25122.0 31 33303.0 32 42660.0 33 57557.0 34 77387.0 35 104919.0 36 157022.0 37 283941.0 38 1327127.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.02217941006859 19.652725866935047 13.086622028136524 25.238472694859844 2 14.224984299792858 20.72988616742144 42.43616832743407 22.608961205351637 3 18.769779232612173 25.684656397004822 30.210206680553185 25.33535768982982 4 12.264840448989368 17.57844985369225 38.66863923628326 31.48807046103512 5 12.261627508354731 38.764494989372736 36.293573436487456 12.68030406578508 6 30.887470907937576 37.765154947695066 18.376723697791768 12.97065044657559 7 27.168636651125126 31.753750985065892 23.02143742052623 18.056174943282745 8 24.195463897476923 38.750505521510355 20.78885664846421 16.265173932548514 9 26.623526151488004 14.583282689415253 20.745855633231663 38.04733552586509 10 15.477626407120232 29.00564903330857 34.92146557519499 20.59525898437621 11 33.47722252892849 21.67920724508473 26.371547952186823 18.47202227379995 12 24.29266330592138 24.25460629112069 32.72978414467854 18.72294625827939 13 29.69809306116099 22.349298443795067 25.87617183840077 22.07643665664317 14 20.031349597060014 23.691174209012633 27.834942206085373 28.442533987841976 15 22.179055039336536 33.43565899968007 23.239930783248695 21.14535517773469 16 20.46758999244825 27.94682930256543 30.491314601394315 21.09426610359201 17 20.703792127818556 29.560294518583717 28.999113156698186 20.736800196899537 18 20.610854329977997 26.722800102516064 32.79183936793545 19.874506199570494 19 23.502532822409027 26.209232694442285 28.49270554216186 21.795528940986827 20 23.660998345635022 27.515306411017786 29.16481080851786 19.658884434829332 21 21.8085675173854 26.628138496385056 28.13403349202379 23.42926049420575 22 22.96941529553372 29.25073306785584 27.811065568699817 19.968786067910628 23 20.755634071051023 28.948268441690317 29.341975143895162 20.9541223433635 24 23.144617682343505 26.87069314061875 29.892514929709403 20.09217424732834 25 21.848380058203194 27.80055375223255 29.435651710525267 20.915414479038986 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 329.0 1 329.0 2 611.5 3 894.0 4 894.0 5 894.0 6 2297.5 7 3701.0 8 3701.0 9 3701.0 10 4680.5 11 5660.0 12 5660.0 13 5660.0 14 7993.0 15 10326.0 16 10326.0 17 10326.0 18 19041.5 19 27757.0 20 27757.0 21 27757.0 22 45541.5 23 63326.0 24 63326.0 25 63326.0 26 95258.0 27 127190.0 28 127190.0 29 127190.0 30 166822.5 31 206455.0 32 206455.0 33 206455.0 34 224862.0 35 243269.0 36 243269.0 37 243269.0 38 250689.0 39 258109.0 40 258109.0 41 258109.0 42 267702.5 43 277296.0 44 277296.0 45 277296.0 46 290060.5 47 302825.0 48 302825.0 49 302825.0 50 282095.5 51 261366.0 52 261366.0 53 261366.0 54 223803.5 55 186241.0 56 186241.0 57 186241.0 58 157437.5 59 128634.0 60 128634.0 61 128634.0 62 105901.5 63 83169.0 64 83169.0 65 83169.0 66 63722.5 67 44276.0 68 44276.0 69 44276.0 70 32270.5 71 20265.0 72 20265.0 73 20265.0 74 14144.0 75 8023.0 76 8023.0 77 8023.0 78 5407.0 79 2791.0 80 2791.0 81 2791.0 82 1849.0 83 907.0 84 907.0 85 907.0 86 570.0 87 233.0 88 233.0 89 233.0 90 143.0 91 53.0 92 53.0 93 53.0 94 32.0 95 11.0 96 11.0 97 11.0 98 5.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1600897174060782 2 0.01754226271327989 3 0.0 4 0.0030047200617204853 5 0.10653500101188366 6 0.039370670220484585 7 0.0250540628675811 8 0.02611455230112951 9 0.025495933464892942 10 0.026291300540054248 11 0.031682121827258644 12 0.03141699946887154 13 0.018646939206559483 14 0.010516520216021698 15 0.003093094181182852 16 0.002430288285215098 17 0.001016302373817223 18 0.002032604747634446 19 7.953670751613049E-4 20 8.837411946236721E-5 21 0.0 22 6.186188362365705E-4 23 0.0 24 4.4187059731183604E-5 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2263106.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.17246973415964 #Duplication Level Percentage of deduplicated Percentage of total 1 81.60607661608464 50.73651328537034 2 12.261551229121832 15.2466184537285 3 3.215072254107084 5.996669472348272 4 1.131584039362305 2.8141349775564404 5 0.5311395865091616 1.6511129933427455 6 0.2955374258820895 1.1024574999579395 7 0.17472652421052692 0.7604225676763727 8 0.1159132167323353 0.5765288767264163 9 0.09771600273165738 0.5467720700139327 >10 0.48931651524247816 5.520795414326228 >50 0.04042232466226311 1.7361947761993526 >100 0.03206328646200814 4.259439931485921 >500 0.004689156854756022 2.0477344446439174 >1k 0.0036944872188984933 4.494829670900881 >5k 4.973348179286432E-4 2.5097755657227143 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 9750 0.43082383237904015 No Hit GTCCTACAGTGGACATTTCTAAATT 9046 0.3997161423282869 No Hit CTGTAGGACGTGGAATATGGCAAGA 8988 0.3971532928638782 No Hit GTCCTAAAGTGTGTATTTCTCATTT 8501 0.3756341947747918 No Hit CTTTAGGACGTGAAATATGGCGAGG 7933 0.3505359448474795 No Hit GGTATCAACGCAGAGTACTTTTTTT 6384 0.28209018932387614 No Hit TATCAACGCAGAGTACTTTTTTTTT 6216 0.2746667632890373 No Hit CTGAAGGACCTGGAATATGGCGAGA 4589 0.20277441710640157 No Hit GTACATGGGAAGCAGTGGTATCAAC 4312 0.19053460156086371 No Hit GTCCTACAGTGTGCATTTCTCATTT 3981 0.17590868478984192 No Hit ATTTAGAAATGTCCACTGTAGGACG 3614 0.15969203386849753 No Hit TTTCTAAATTTTCCACCTTTTTCAG 3460 0.15288722666989527 No Hit GTCCTTCAGTGTGCATTTCTCATTT 3369 0.14886620423435756 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 3141 0.1387915546156477 No Hit CTGTAGGACCTGGAATATGGCGAGA 3020 0.1334449203881745 No Hit GAATATGGCAAGAAAACTGAAAATC 2989 0.1320751215365078 No Hit CCCATGTACTCTGCGTTGATACCAC 2967 0.13110300622242174 No Hit GGAATATGGCGAGAAAACTGAAAAT 2649 0.11705152122790537 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 2628 0.1161235929735505 No Hit ATTCCAGGTCCTTCAGTGTGCATTT 2306 0.10189535974010938 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 4.4187059731183604E-5 0.0 4 0.0 0.0 0.0 4.4187059731183604E-5 0.0 5 0.0 0.0 0.0 4.4187059731183604E-5 0.0 6 0.0 0.0 0.0 4.4187059731183604E-5 0.0 7 0.0 0.0 0.0 4.4187059731183604E-5 0.0 8 0.0 0.0 0.0 4.4187059731183604E-5 0.0 9 0.0 0.0 0.0 4.4187059731183604E-5 0.0 10 0.0 0.0 0.0 8.837411946236721E-5 0.0 11 0.0 0.0 0.0 8.837411946236721E-5 0.0 12 0.0 0.0 0.0 8.837411946236721E-5 0.0 13 0.0 0.0 0.0 8.837411946236721E-5 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACGACGT 40 0.0052545033 14.260437 5 TAGGACC 1160 0.0 13.358323 4 TAGAGCG 55 0.0030524065 12.099764 5 GGCGAGG 2150 0.0 11.70312 19 ACCTTTT 990 0.0 11.7014065 15 CCAACGT 120 1.2854616E-7 11.077482 19 TGTAGGA 4650 0.0 10.87798 2 AGGACCT 2830 0.0 10.850697 5 GCGATAA 115 8.846255E-7 10.736782 11 ATTTAGA 1180 0.0 10.725909 1 CCACCTT 1070 0.0 10.650464 13 GGACCTG 2820 0.0 10.610555 6 TAGAAAT 1215 0.0 10.484575 4 CTGTAGG 4710 0.0 10.465821 1 CACCTTT 1100 0.0 10.445868 14 AAATGTC 1130 0.0 10.4227295 7 ATTGCGA 110 6.0830116E-6 10.358623 17 TTCCACC 1165 0.0 10.353959 11 TGGCGAG 4815 0.0 10.353245 18 GTAGGAC 4755 0.0 10.336234 3 >>END_MODULE