FastQCFastQC Report
Thu 26 May 2016
SRR1512652_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512652_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2360376
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT60960.2582639376099401No Hit
GGTATCAACGCAGAGTACTTTTTTT38310.16230464976766415No Hit
TATCAACGCAGAGTACTTTTTTTTT34780.1473494053489783No Hit
GTACATGGGAAGCAGTGGTATCAAC26990.11434618891227499No Hit
GTCCTACAGTGGACATTTCTAAATT25010.10595769487573166No Hit
GTCCTAAAGTGTGTATTTCTCATTT24740.10481380932529394No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCCAT402.8035234E-416.59240510
TATACCG707.3728024E-613.5523255
GTATTAG1551.7462298E-1011.4041071
TCGCGCG752.1579665E-411.3517799
GACCGTA600.005939107411.0677337
ATCGCGC700.00154969610.8077748
GTACCGC803.821634E-410.6724566
GTCCTAC14200.010.6500811
TAGGACC5050.010.5198254
GGCGAGG6800.010.45339119
GCACCGT1201.5522655E-610.2771816
CCGTCCA1201.609651E-610.2481359
TGTACCG1409.895848E-810.1642445
ACCGTGC1401.0362055E-710.1322888
CGTAGAC750.002679557310.1190693
GTGTTAT1751.4515535E-910.1007821
ACCGTAT750.002750457710.08725558
GTGCTAC2052.1827873E-1110.0597211
GGTATCA21350.09.9811921
CGCGCGC1054.196132E-59.93286910
Produced by FastQC (version 0.11.5)