FastQCFastQC Report
Thu 26 May 2016
SRR1512652_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512652_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2360376
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT74930.31744942331221804No Hit
GGTATCAACGCAGAGTACTTTTTTT51370.21763481750365193No Hit
TATCAACGCAGAGTACTTTTTTTTT48480.2053910055008185No Hit
GTACATGGGAAGCAGTGGTATCAAC28950.12264995068582295No Hit
GTCCTAAAGTGTGTATTTCTCATTT26940.11413435825478652No Hit
CTGTAGGACGTGGAATATGGCAAGA26640.11286337430985573No Hit
GTCCTACAGTGGACATTTCTAAATT25210.10680501750568554No Hit
CTTTAGGACGTGAAATATGGCGAGG24980.10583059648123859No Hit
ACGCAGAGTACTTTTTTTTTTTTTT24930.1056187658237501No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC4900.012.6105384
GGCGTAG550.003027217712.1131931
GTACCGA855.327879E-511.1759476
TTATACT3100.010.426384
CCTAGAC1752.3283064E-1010.3210213
TGTAGGA16400.010.0291612
GTAGGAC15750.09.8985233
GACGTGG7800.09.8640867
ATAGGAC2350.09.7084633
GGTATCA21950.09.6692681
TATACTG3350.09.6484965
CTGTAGG17100.09.6288241
AGGACGT16150.09.5949045
GTATTAG1804.0527084E-99.51750851
GTCTTAC1606.452501E-89.51750851
CTAGACT1502.6235648E-79.5064064
CTAGACA2002.6193447E-109.5064064
TAAGACG900.00110711679.5064064
GTAAGAC1606.571827E-89.5062053
GTCCTAA9350.09.4666131
Produced by FastQC (version 0.11.5)