FastQCFastQC Report
Thu 26 May 2016
SRR1512651_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512651_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2175583
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT67210.30892868716109656No Hit
GTATCAACGCAGAGTACTTTTTTTT64810.29789716135858757No Hit
CTGTAGGACGTGGAATATGGCAAGA62730.2883365056630797No Hit
GTCCTAAAGTGTGTATTTCTCATTT58730.2699506293255647No Hit
CTTTAGGACGTGAAATATGGCGAGG48190.22150384517621255No Hit
GGTATCAACGCAGAGTACTTTTTTT40540.186340856680715No Hit
GTCCTACAGTGTGCATTTCTCATTT39820.18303139893996231No Hit
TATCAACGCAGAGTACTTTTTTTTT36960.16988549735863903No Hit
CTGTAGGACCTGGAATATGGCGAGA27140.12474817095003961No Hit
CTGAAGGACCTGGAATATGGCGAGA25570.11753171448756494No Hit
ATTTAGAAATGTCCACTGTAGGACG22070.10144407269223929No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC11650.013.5985814
CTACCGT604.142809E-412.6485814
TCCAACG2000.011.84379918
GGCGAGG16800.011.508594519
CCACCTT8550.011.42942413
TGTAGGA39750.011.360122
GTAGGAC38350.011.1811413
AGGACCT22400.011.1804425
GGTATCA13900.011.1690851
CCTACCG600.005938729311.0677633
CGTCCAA600.005993123611.05421218
GTCCTAA18350.011.0307861
CGGTTTC1303.331661E-810.94715213
GGACCTG22200.010.8965816
CACCTTT8250.010.81328914
CTGTAGG37000.010.8085621
TAGGACA10550.010.7902584
GTCCTAT3400.010.6929641
AGGACGT37800.010.6408695
GGACGTG37250.010.568786
Produced by FastQC (version 0.11.5)