FastQCFastQC Report
Thu 26 May 2016
SRR1512651_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512651_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2175583
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT82650.3798981698239047No Hit
GTCCTACAGTGGACATTTCTAAATT71280.3276363163345182No Hit
CTGTAGGACGTGGAATATGGCAAGA70410.32363738823110866No Hit
GTCCTAAAGTGTGTATTTCTCATTT61640.28332635436110687No Hit
CTTTAGGACGTGAAATATGGCGAGG57550.26452679580599775No Hit
GGTATCAACGCAGAGTACTTTTTTT53210.24457811997979392No Hit
TATCAACGCAGAGTACTTTTTTTTT52080.23938410991444595No Hit
GTCCTACAGTGTGCATTTCTCATTT42460.19516607732272223No Hit
CTGTAGGACCTGGAATATGGCGAGA33310.15310838520065656No Hit
CTGAAGGACCTGGAATATGGCGAGA30100.13835371943980074No Hit
ACGCAGAGTACTTTTTTTTTTTTTT25740.11831311423190934No Hit
ATTTAGAAATGTCCACTGTAGGACG25130.1155092680904383No Hit
TTTCTAAATTTTCCACCTTTTTCAG23230.10677597683011865No Hit
GTCCTTCAGTGTGCATTTCTCATTT23060.10599457708577426No Hit
GAATATGGCAAGAAAACTGAAAATC22980.10562685955902394No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGACG551.9488335E-413.8280894
TAGGACC10250.012.6139154
GGTATCA16050.012.3878541
ACCGTGC1157.09133E-811.56366358
GCCTAAC752.0480377E-411.41579151
CCAACGT752.0851288E-411.39375419
GTTATGC855.2503307E-511.1919541
GGCGAGG17150.011.1280119
AATCCCG1201.2857163E-711.0772619
TGTAGGA36350.011.0116542
CTGTAGG35750.010.8303671
GCGCCAC803.782286E-410.68434413
AGGACCT20100.010.5478445
GTAGGAC35600.010.5213413
AGGACGT36500.010.31471355
GGACGTG36150.010.2752796
ACGTGGC750.002651251410.1319728
GACGTGG18100.010.1284737
TTAGGAC22100.010.0658043
GTCCTAA18550.010.05164051
Produced by FastQC (version 0.11.5)