FastQCFastQC Report
Thu 26 May 2016
SRR1512650_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512650_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1872527
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT66200.35353295306289306No Hit
GTCCTAAAGTGTGTATTTCTCATTT53710.2868316451511781No Hit
GTCCTACAGTGGACATTTCTAAATT51810.27668492897565694No Hit
CTGTAGGACGTGGAATATGGCAAGA48090.25681872677937356No Hit
CTTTAGGACGTGAAATATGGCGAGG44090.23545721904143438No Hit
GGTATCAACGCAGAGTACTTTTTTT42820.2286749403346387No Hit
TATCAACGCAGAGTACTTTTTTTTT39120.20891554567704496No Hit
GTCCTACAGTGTGCATTTCTCATTT30190.1612259796520958No Hit
CTGAAGGACCTGGAATATGGCGAGA23730.12672714465532406No Hit
ATTTAGAAATGTCCACTGTAGGACG22670.12106634510477018No Hit
CTGTAGGACCTGGAATATGGCGAGA21130.1128421646256636No Hit
GTCCTTCAGTGTGCATTTCTCATTT21090.11262854954828422No Hit
GTACTTTTTTTTTTTTTTTTTTTTT20220.10798242161528245No Hit
ACGCAGAGTACTTTTTTTTTTTTTT19430.10376352383703946No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC10500.014.0952464
GTGTAGG3300.012.4962551
GTGACGA550.003169910512.0387019
GGTATCA14750.011.9153161
GTCGAGG802.934232E-511.84815919
GTCCTAA15250.011.71781
TATGTCG1251.8559149E-811.3901316
TCCTATA2700.011.243772
ACCTTTT8550.011.21130115
GGCGAGG12950.011.19856919
AATGTCC8900.011.1570788
AGGACCT20100.011.0919845
GTCTAGA1603.0559022E-1011.0457971
AAATGTC9150.010.990627
CCACCTT9100.010.94792913
GGACCTG19750.010.8562226
TGTAGGA32500.010.8296962
GTATAAT2450.010.8203731
GTAGGAC31850.010.7826343
ATGTCCA9500.010.7533659
Produced by FastQC (version 0.11.5)