FastQCFastQC Report
Thu 26 May 2016
SRR1512650_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512650_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1872527
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT81960.43769729355037335No Hit
GGTATCAACGCAGAGTACTTTTTTT58160.31059632250963537No Hit
TATCAACGCAGAGTACTTTTTTTTT56200.3001291837180452No Hit
GTCCTAAAGTGTGTATTTCTCATTT55200.2947888067835604No Hit
CTGTAGGACGTGGAATATGGCAAGA53920.28795312430741987No Hit
GTCCTACAGTGGACATTTCTAAATT52170.2786074646720715No Hit
CTTTAGGACGTGAAATATGGCGAGG51470.27486920081793215No Hit
GTCCTACAGTGTGCATTTCTCATTT31000.16555168496902847No Hit
CTGAAGGACCTGGAATATGGCGAGA29060.155191353716128No Hit
ACGCAGAGTACTTTTTTTTTTTTTT26750.14285508299746813No Hit
ATTTAGAAATGTCCACTGTAGGACG25020.13361623090080943No Hit
CTGTAGGACCTGGAATATGGCGAGA24690.13185390651242945No Hit
TTTCTAAATTTTCCACCTTTTTCAG22560.12047890364197686No Hit
GTACTTTTTTTTTTTTTTTTTTTTT22090.117968926482769No Hit
GTCCTTCAGTGTGCATTTCTCATTT21480.11471129655273328No Hit
GAATATGGCAAGAAAACTGAAAATC19950.10654051984297155No Hit
GAGTACTTTTTTTTTTTTTTTTTTT19080.10189439190996978No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC10100.012.7995294
TGGCGAA1103.8382495E-812.08567618
GGTATCA15500.012.0356641
TAGAAAT9950.011.3684264
TGCGGTA600.00588836111.08031112
GACGTGG15600.011.0203137
TAGCGAG700.001497046710.85244318
GGCGAGG14100.010.775187519
AGGACGT34450.010.7612695
GTCCTAT2750.010.7293811
GGACGTG33950.010.7160266
AATGTCC9350.010.666118
ACCTTTT8800.010.57496615
AAATGTC10000.010.4479887
CCACCTT8550.010.4407613
CACCTTT8700.010.369349514
TGTAGGA34950.010.3639172
TTTAGAA11300.010.2642572
GAAATGT10200.010.2439466
CTGTAGG34850.010.2144381
Produced by FastQC (version 0.11.5)