FastQCFastQC Report
Thu 26 May 2016
SRR1512649_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512649_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1898227
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT62400.3287278075804422No Hit
GGTATCAACGCAGAGTACTTTTTTT42970.2263691328803141No Hit
TATCAACGCAGAGTACTTTTTTTTT38010.20023948663674052No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCTAA801.373146E-613.5068981
GGTATCA13250.013.0481741
TAGGACC3500.011.6539224
GGCGAGG4750.011.37386319
GTCCTAA6100.011.2725231
GTCTTAG1603.0377123E-1011.0510981
GTCCTAT2100.010.758741
TGGCGAG9900.010.62428318
TTAGACT1201.5535024E-610.276234
TCTATAC1951.6370905E-1110.2154243
GCACTCG750.00267766510.11974213
ATTAGAC750.002681197410.11813453
TGCGTTG2250.010.11679512
TAGGACA4150.010.0571814
TATGGCG11050.010.04799416
TTAGGAC6800.010.04373553
GTATCAA27400.09.9666021
GTAGGAC11350.09.9454133
TGTAGGA11550.09.9374532
GTCCAGA3200.09.8231981
Produced by FastQC (version 0.11.5)