FastQCFastQC Report
Thu 26 May 2016
SRR1512649_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512649_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1898227
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT78610.41412328451760516No Hit
GGTATCAACGCAGAGTACTTTTTTT55780.2938531587634145No Hit
TATCAACGCAGAGTACTTTTTTTTT55710.2934843935946544No Hit
ACGCAGAGTACTTTTTTTTTTTTTT26310.1386030227154076No Hit
GTACTTTTTTTTTTTTTTTTTTTTT20780.10947057438335879No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17300.013.4739231
TAGACCG600.00585025111.08999355
ACCGTCC600.00588363411.0815198
TGGACCG803.7506217E-410.6939225
TCTATTC1701.382432E-1010.6234683
TAGGACT2450.010.4754044
GGATTAT1659.058567E-1010.379171
GGCGAGG5400.010.37465119
CTAGGAC2700.010.2092973
TAAGACT2053.6379788E-1210.200984
TAGGACC3750.010.1391554
TCGAGAC750.002636019810.1388883
GTTACCC951.6351884E-410.0054833
TTAGGAC7650.09.9400863
GACGTGA6050.09.73420057
TGGCGAG10850.09.53945318
GTATAAC1201.6705835E-59.5142391
GATCTAT1106.725699E-59.5142391
GTCTTAG2106.548362E-119.5142391
GAGGTTA1002.755711E-49.4999476
Produced by FastQC (version 0.11.5)