FastQCFastQC Report
Thu 26 May 2016
SRR1512648_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512648_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2425821
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT60340.24874052949496275No Hit
GGTATCAACGCAGAGTACTTTTTTT37260.15359748307892462No Hit
TATCAACGCAGAGTACTTTTTTTTT35050.14448716537617573No Hit
GTCCTACAGTGGACATTTCTAAATT26550.10944748190406466No Hit
GTACATGGGAAGCAGTGGTATCAAC25810.10639696828413968No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTATA500.001549847413.2423779
GTATTAG2000.011.2950331
GCCCTAT1404.964022E-911.2248771
ACGGCGT600.0060814811.0325778
TAATACT2100.010.8428384
GTCTTAT2550.010.7846851
TAAGACG803.818531E-410.6734194
CCTATAC1409.883479E-810.1651613
GGTATCA21250.09.8448771
GTATTAA2700.09.8217681
GTCCTAT1603.9684892E-89.8217681
GTATTGG2600.09.8217671
TAGGACC5950.09.7266644
TAGCACA2650.09.6664924
GTCCTAC16100.09.6387541
TATGTCG800.00454998069.49161716
CCTAGAC1502.7007263E-79.4874833
ATACCGT800.00456635939.4874836
CTAATAG1701.7018465E-89.4874833
GTCTAGA2700.09.4579981
Produced by FastQC (version 0.11.5)