FastQCFastQC Report
Thu 26 May 2016
SRR1512648_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512648_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2425821
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT77210.31828399539784674No Hit
GGTATCAACGCAGAGTACTTTTTTT50290.20731125668381964No Hit
TATCAACGCAGAGTACTTTTTTTTT49350.2034362799233744No Hit
GTACATGGGAAGCAGTGGTATCAAC29240.12053651114406216No Hit
CTGTAGGACGTGGAATATGGCAAGA28720.11839290697870947No Hit
GTCCTACAGTGGACATTTCTAAATT28180.11616685649930478No Hit
GTCCTAAAGTGTGTATTTCTCATTT25700.10594351355685354No Hit
ACGCAGAGTACTTTTTTTTTTTTTT25240.10404724833365693No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGACCG907.4139352E-611.6183115
TCCAACG1406.85759E-1011.53073318
GTCTTAG2400.011.0993671
TAGGACC5600.010.8634274
GTCCTAC14250.010.748861
GGACGTG16800.010.5174346
TAGGACA4200.010.4107854
GACGTGG8250.010.3619387
TTAGGAC10200.010.3437923
AGGACGT17450.010.2957795
CGTGGAA8350.010.2378439
TAGGACG17500.010.2116224
GTAGGAC15000.010.1387813
ACCGTCT856.605724E-410.0571768
GACGTGA9100.010.0203357
TAAGCTG2002.5465852E-119.9811855
TGTAGGA16600.09.9067412
GGTATCA24200.09.867561
CTAGGAC3100.09.8117233
GTCTAGA1752.5120244E-99.7855641
Produced by FastQC (version 0.11.5)