FastQCFastQC Report
Thu 26 May 2016
SRR1512647_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512647_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1860853
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT67030.36021115047776475No Hit
GGTATCAACGCAGAGTACTTTTTTT42380.22774501801055752No Hit
TATCAACGCAGAGTACTTTTTTTTT39770.21371919221991206No Hit
GTACATGGGAAGCAGTGGTATCAAC32780.1761557737231259No Hit
CCCATGTACTCTGCGTTGATACCAC21620.11618327723898662No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC4300.015.0016514
CGCGCGC907.6082197E-611.59096810
GGCGAGG5800.011.4382119
TTAGGAC7900.011.2878473
ATAATAC1803.6379788E-1211.0676943
ACCGTGC600.00606380411.0366748
GTCCTAA6150.010.8547751
GACGTGA6850.010.8022557
TGGCGAG11800.010.59923318
ATCGCGC909.9321915E-510.5111178
GTAGGAC13000.010.5082283
TAATACT2100.010.38984
GTACTGG5300.010.3728761
GCGCGCC1201.5575952E-610.2740911
TGTAGGA13600.010.2538932
GTATTAG1451.0011536E-710.155711
TATGGCG12400.010.1019116
GTTTAGA1853.5652192E-1010.0825161
ACTATCG3200.010.07651111
CTAGGAC2002.5465852E-119.9609253
Produced by FastQC (version 0.11.5)