FastQCFastQC Report
Thu 26 May 2016
SRR1512647_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512647_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1860853
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT81350.43716510653985025No Hit
GGTATCAACGCAGAGTACTTTTTTT54560.2931988717002364No Hit
TATCAACGCAGAGTACTTTTTTTTT53030.28497683589192696No Hit
GTACATGGGAAGCAGTGGTATCAAC34900.18754839850326704No Hit
CCCATGTACTCTGCGTTGATACCAC26690.14342884687828647No Hit
ACGCAGAGTACTTTTTTTTTTTTTT26210.1408493846639149No Hit
GTACTTTTTTTTTTTTTTTTTTTTT21400.11500102372406633No Hit
CTGTAGGACGTGGAATATGGCAAGA19820.10651029393509322No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGTAC400.00522312514.2731261
AATCCCG658.0623315E-411.68579319
GGACCGC600.005876087611.0834116
ACCGTCA600.005880792611.082228
TAGGACC4800.010.4958654
GTATTAC1002.3565173E-510.4669591
TTAGTAC1201.5121714E-610.297833
GTAGGAC12700.010.25416853
CTAGGGT1409.604446E-810.1846684
CGTGGAA6400.010.0927369
TGCACCG856.556423E-410.0651195
GTCTTAG1909.640644E-1110.0162281
GTACTAG1151.0068925E-59.9291311
GTATAAT2300.09.9291311
GACGTGG6700.09.9243767
GTCATAC1252.5172958E-69.8960331
GTAGAAC2500.09.8859173
GAACCGG1252.5821737E-69.87635213
AACCGGG1252.5848349E-69.87555614
TAGGGTG1356.387836E-79.8580175
Produced by FastQC (version 0.11.5)