FastQCFastQC Report
Thu 26 May 2016
SRR1512646_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512646_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2667044
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT76930.2884466847941016No Hit
GTCCTACAGTGGACATTTCTAAATT57360.21506956765617666No Hit
CTGTAGGACGTGGAATATGGCAAGA51020.191297931342715No Hit
GTCCTAAAGTGTGTATTTCTCATTT49010.18376149774806866No Hit
GGTATCAACGCAGAGTACTTTTTTT47350.17753737846094778No Hit
TATCAACGCAGAGTACTTTTTTTTT45350.17003843956080214No Hit
CTTTAGGACGTGAAATATGGCGAGG41900.15710276995805095No Hit
GTCCTACAGTGTGCATTTCTCATTT32030.12009550648583225No Hit
GTACATGGGAAGCAGTGGTATCAAC28660.10745979443908688No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTAT751.5454476E-512.6130568
TAGGACC11650.012.5408664
TTAGACG701.1054102E-412.1976794
GTCTAAT1653.6379788E-1211.902121
CGCCCTA1850.011.28534116
GCACCGT1105.060865E-711.2120086
TCCGGAC600.00593566411.0686813
GTATAAA3400.010.9744561
AGGACCT21400.010.9057125
AAATGTC7950.010.8594297
GGACCTG21050.010.81662756
CTAGCAC1951.8189894E-1210.703783
CTAGGAC4000.010.673373
TGTAGGA34500.010.3399362
GTCCTAC31150.010.2763241
CACCTTT7850.010.27573914
TAGGACT3700.010.2563064
GTCCTAA16950.010.2537291
ATTTAGA8550.010.2212071
TAGGACA9950.010.202194
Produced by FastQC (version 0.11.5)