FastQCFastQC Report
Thu 26 May 2016
SRR1512646_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512646_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2667044
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT98560.3695477089991766No Hit
GGTATCAACGCAGAGTACTTTTTTT66860.2506895274318684No Hit
TATCAACGCAGAGTACTTTTTTTTT65710.24637763756428466No Hit
CTGTAGGACGTGGAATATGGCAAGA60170.22560557681088125No Hit
GTCCTACAGTGGACATTTCTAAATT58940.22099372938729167No Hit
GTCCTAAAGTGTGTATTTCTCATTT51680.19377258117976306No Hit
CTTTAGGACGTGAAATATGGCGAGG48530.1819617524120337No Hit
GTCCTACAGTGTGCATTTCTCATTT34210.12826934988699099No Hit
GTACATGGGAAGCAGTGGTATCAAC31980.11990803301332861No Hit
ACGCAGAGTACTTTTTTTTTTTTTT31340.11750837256528202No Hit
CTGAAGGACCTGGAATATGGCGAGA27260.10221053720898493No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC11000.011.6657144
TTGCGAG951.3698185E-510.99366118
TGTAGGA33850.010.5883012
TGCGTTT1106.063854E-610.36154912
GGCGAGG13250.010.31857919
TAGGACA10400.010.2365814
TAAGACG1303.8144935E-710.2365814
CTGTAGG34050.010.1464561
GCACCGG750.002648525910.1333846
ACGGTTG750.002661329210.12749419
GTAGGAC34300.010.087173
TGGCGAG31250.09.96545418
TGCACCG1054.079973E-59.9584085
GGACCTG23000.09.9543976
ATTGCGA1054.1332984E-59.94664617
AGGACCT22950.09.8578185
GATATAC4650.09.8245611
TTTAGAA9800.09.60401252
GGACGTG34050.09.5139976
AGGACGT34400.09.5057535
Produced by FastQC (version 0.11.5)