FastQCFastQC Report
Thu 26 May 2016
SRR1512645_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512645_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2608228
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT76110.29180731132401No Hit
GTCCTACAGTGGACATTTCTAAATT48840.1872535683230147No Hit
GGTATCAACGCAGAGTACTTTTTTT48550.18614170233583874No Hit
TATCAACGCAGAGTACTTTTTTTTT44860.17199416615418592No Hit
CTGTAGGACGTGGAATATGGCAAGA41020.1572715268757179No Hit
GTCCTAAAGTGTGTATTTCTCATTT40590.15562289799818113No Hit
GTACATGGGAAGCAGTGGTATCAAC36870.14136034119716528No Hit
CTTTAGGACGTGAAATATGGCGAGG34320.13158358855130764No Hit
CCCATGTACTCTGCGTTGATACCAC26850.10294345432991289No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC8500.013.2810774
ACCGCGC500.001547508513.2450958
CCCGTAC1658.185452E-1110.92909416
TATGTCG1408.483767E-910.84692116
CCTACAC3000.010.7515553
GTGTAGG4200.010.74821951
CTAGTAC1252.2856693E-710.6250673
CCCCGTA1803.6379788E-1110.544600515
GTCCTAC23900.010.4295071
AGGACCT17550.010.3783765
GGCGAGG12500.010.23731519
GTAGGAC29100.010.2364723
AATGTCC8050.010.2246968
TGTAGGA30100.010.2115622
TTAGGAC16550.010.2031823
GGACGTG29050.010.1887786
CAAGTCG2053.6379788E-1210.1808146
AGGACGT29700.010.1253055
TAGAAAT8100.010.072074
GTCCTAA13500.010.0316721
Produced by FastQC (version 0.11.5)