FastQCFastQC Report
Thu 26 May 2016
SRR1512645_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512645_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2608228
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT96370.3694845696005104No Hit
TATCAACGCAGAGTACTTTTTTTTT70300.26953165137403634No Hit
GGTATCAACGCAGAGTACTTTTTTT68850.26397232143815647No Hit
CTGTAGGACGTGGAATATGGCAAGA47090.18054403219350457No Hit
GTCCTACAGTGGACATTTCTAAATT44840.17191748574127722No Hit
GTACATGGGAAGCAGTGGTATCAAC41790.1602237227727024No Hit
CTTTAGGACGTGAAATATGGCGAGG40050.15355252684964657No Hit
GTCCTAAAGTGTGTATTTCTCATTT38190.14642124844913865No Hit
ACGCAGAGTACTTTTTTTTTTTTTT32810.1257942173767017No Hit
CCCATGTACTCTGCGTTGATACCAC26780.10267507288473246No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGAGG12000.011.3157519
TAGGACC7700.010.8624724
TTAGGAC16600.010.76431753
GAACCGC803.7714682E-410.6878236
CGCCCTA1909.094947E-1210.49528616
AGGACCG1106.012442E-610.3689215
TAGGACA7600.010.2550314
CGCAACG750.002658485410.12878317
AGGACCT15400.010.1220435
GACGTGA14550.010.1192237
TTATACT2950.09.9879514
TGGCGAG27900.09.90415418
CACCTTT7900.09.85746714
AGGACGT27100.09.8555775
CTAGACA3000.09.82148554
GGACGTG27000.09.7465926
TGTAGGA27850.09.7281752
CTTATAC2850.09.67141153
ACCTTTT7500.09.62271315
TATGGCG29450.09.51185616
Produced by FastQC (version 0.11.5)