##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512645_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2608228 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.08014981819074 31.0 31.0 34.0 28.0 34.0 2 31.237222742797027 33.0 31.0 34.0 28.0 34.0 3 31.322889333294484 33.0 31.0 34.0 28.0 34.0 4 34.84302445951811 37.0 35.0 37.0 32.0 37.0 5 34.567404383359126 37.0 35.0 37.0 32.0 37.0 6 34.434817431604905 37.0 35.0 37.0 31.0 37.0 7 34.38694968384666 37.0 35.0 37.0 30.0 37.0 8 34.36237859573626 37.0 35.0 37.0 30.0 37.0 9 35.878413236879595 39.0 37.0 39.0 30.0 39.0 10 35.6427743280112 39.0 37.0 39.0 30.0 39.0 11 35.76835077301524 39.0 37.0 39.0 30.0 39.0 12 35.67867111310821 39.0 37.0 39.0 30.0 39.0 13 35.66751104581348 39.0 37.0 39.0 30.0 39.0 14 36.81256201528394 40.0 37.0 41.0 30.0 41.0 15 36.77394844315758 40.0 37.0 41.0 30.0 41.0 16 36.72194953815387 40.0 37.0 41.0 30.0 41.0 17 36.67958245981563 40.0 37.0 41.0 30.0 41.0 18 36.601167152564884 40.0 37.0 41.0 30.0 41.0 19 36.61957543588981 40.0 37.0 41.0 30.0 41.0 20 36.57159113390394 40.0 37.0 41.0 30.0 41.0 21 36.51433003556438 40.0 37.0 41.0 30.0 41.0 22 36.40344095684886 40.0 37.0 41.0 30.0 41.0 23 36.329692802929806 40.0 37.0 41.0 30.0 41.0 24 36.26387071989105 40.0 37.0 41.0 30.0 41.0 25 36.16729519045114 40.0 37.0 41.0 30.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.7469871671314579 1101 2 0.7683453559007276 1101 3 0.7123785768256674 1101 4 0.7323568395542281 1101 5 0.7815702531543991 1101 6 0.9633186289113596 1101 7 0.8881443990222095 1101 8 0.990049073633493 1101 9 1.0477987600556347 1101 10 1.096005172813733 1101 11 1.1130821884668478 1101 12 1.1725930130111664 1101 13 1.057100890253487 1101 14 1.2189543269376344 1101 15 1.2880761509830947 1101 16 1.2428684518786568 1101 17 1.3566890799354496 1101 18 1.3622832952546702 1101 19 1.3159586877863418 1101 20 1.3028598097248718 1101 21 1.2178704839624928 1101 22 1.3273926954160302 1101 23 1.3727686238977626 1101 24 1.335910818440908 1101 25 1.2935481179547565 1102 1 0.579073031262503 1102 2 0.5453490073198743 1102 3 0.6156879778752824 1102 4 0.5698169632811556 1102 5 0.6267562825425159 1102 6 0.7095713676206046 1102 7 0.7051290761595084 1102 8 0.7589179508561585 1102 9 0.8045910922203134 1102 10 0.8534486230498501 1102 11 0.8826198598634676 1102 12 0.9006521161025418 1102 13 0.9478593891988609 1102 14 1.126793156814422 1102 15 1.0203969334143252 1102 16 0.9863671958611704 1102 17 1.0586250767966376 1102 18 1.0269483606403114 1102 19 1.0988644188134415 1102 20 1.1711206869753568 1102 21 1.0792469539405047 1102 22 1.1754931084283058 1102 23 1.1757057256602437 1102 24 1.249160708979474 1102 25 1.1623936105631074 1103 1 0.48392155208136245 1103 2 0.5098058340367189 1103 3 0.5173466670534772 1103 4 0.5250976416993396 1103 5 0.5411824445152078 1103 6 0.7312031044088698 1103 7 0.6146733177627794 1103 8 0.6623967409778047 1103 9 0.6380960782960798 1103 10 0.7836330886512215 1103 11 0.7584515028536956 1103 12 0.7335950121810626 1103 13 0.7614992070491127 1103 14 0.8286073226460999 1103 15 0.8819959456255262 1103 16 0.8237125483777206 1103 17 0.8115836136402947 1103 18 0.8976164563887963 1103 19 0.9066261485144835 1103 20 0.8712264716145839 1103 21 0.9013274317286388 1103 22 0.9473846518250326 1103 23 0.9031549193578527 1103 24 0.9786837005551021 1103 25 1.0370126287559671 1104 1 0.4622992385354614 1104 2 0.40046502668191764 1104 3 0.5557473181645491 1104 4 0.3320756877165394 1104 5 0.38399643652533655 1104 6 0.5777728304187377 1104 7 0.4470007532678153 1104 8 0.42444735875000106 1104 9 0.5584748231877796 1104 10 0.7284399619998041 1104 11 0.60289136200889 1104 12 0.605337211935371 1104 13 0.5387473839190093 1104 14 0.6228504919980224 1104 15 0.6270106854199184 1104 16 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1107 16 0.8147229672931076 1107 17 0.8519169229872716 1107 18 0.8228258635849528 1107 19 0.8792921814363908 1107 20 0.8368935384746621 1107 21 0.9347948361651035 1107 22 0.958238856443792 1107 23 0.8771263149976392 1107 24 0.9179439361011177 1107 25 0.8344973437665004 1108 1 0.36512256304367696 1108 2 0.37709383158196275 1108 3 0.4369725117043828 1108 4 0.47024915413343393 1108 5 0.5639384540082801 1108 6 0.6091342264603341 1108 7 0.6149205088879128 1108 8 0.5371633747506337 1108 9 0.6678572659363695 1108 10 0.6739469005138261 1108 11 0.7929676756124806 1108 12 0.6815855835681575 1108 13 0.7751370871265877 1108 14 0.9001118393426069 1108 15 0.8708211392618281 1108 16 1.0036844528059845 1108 17 0.9775306058298625 1108 18 0.9903289838972569 1108 19 1.0019306738145985 1108 20 1.0881981567939434 1108 21 1.0657668439752044 1108 22 1.2088654254370823 1108 23 1.0661120946001148 1108 24 1.1781542020892601 1108 25 1.1490875513202923 1109 1 0.025559607256258943 1109 2 0.014960754068845006 1109 3 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-0.9230106300622296 2315 4 -0.9155265417777017 2315 5 -0.9480026589065957 2315 6 -0.9381216277715936 2315 7 -1.0989918771629164 2315 8 -1.2443623360912994 2315 9 -1.4260098390624663 2315 10 -1.3007456606888894 2315 11 -1.410068570946649 2315 12 -1.4799116773082517 2315 13 -1.5056994993118948 2315 14 -1.7600990650296708 2315 15 -1.826598275124951 2315 16 -1.8233815630586676 2315 17 -1.7467342557480237 2315 18 -1.6061362164741126 2315 19 -1.6326942644261706 2315 20 -1.5126528989495256 2315 21 -1.4338051849308258 2315 22 -1.5689775350967352 2315 23 -1.6836932620288891 2315 24 -1.472010443942132 2315 25 -1.636060643681418 2316 1 -1.691124203855093 2316 2 -1.5099087434417378 2316 3 -1.489036602561935 2316 4 -1.4676789686198646 2316 5 -1.5494705164691709 2316 6 -4.649261009602601 2316 7 -2.604271581005907 2316 8 -2.0699105791280843 2316 9 -2.1572291362771807 2316 10 -1.8307021773183436 2316 11 -1.9365705100699344 2316 12 -2.0088163664609127 2316 13 -1.9426421482718226 2316 14 -2.264600769107709 2316 15 -2.3187313107343073 2316 16 -2.3270482688995457 2316 17 -2.3958040641861515 2316 18 -2.506489959569663 2316 19 -2.4412481485488655 2316 20 -2.5363019763969348 2316 21 -2.7214412173670723 2316 22 -2.711190486089805 2316 23 -2.8250494730995186 2316 24 -2.8650548674936616 2316 25 -2.7886461413897266 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 41223.0 3 2.0 4 806.0 5 6731.0 6 8158.0 7 6231.0 8 5016.0 9 4479.0 10 4102.0 11 3829.0 12 3687.0 13 3464.0 14 3395.0 15 3392.0 16 3467.0 17 3614.0 18 4023.0 19 4442.0 20 5698.0 21 7243.0 22 9131.0 23 11200.0 24 13434.0 25 15805.0 26 18073.0 27 20551.0 28 23655.0 29 28639.0 30 36194.0 31 46746.0 32 60056.0 33 79222.0 34 105879.0 35 143762.0 36 217200.0 37 431863.0 38 1223816.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.51705877926255 19.466167330383595 13.81235112442811 24.204422765925752 2 16.373954840280614 20.79571141800445 38.96754229050312 23.86279145121182 3 19.634556488159777 24.789167204707564 29.99227828242009 25.583998024712564 4 13.835725631968272 17.334014272891896 36.540075555151695 32.29018453998814 5 13.692591165758298 37.819534850882455 34.95513657788631 13.53273740547294 6 32.86972810784709 36.44955771355763 17.225180429778998 13.455533748816281 7 28.60067809141924 31.480553287623586 21.596839715095758 18.321928905861416 8 26.051531656875422 36.37795813086434 19.838272803239455 17.732237409020783 9 26.88312161449595 14.596040458271414 19.647456977134365 38.873380950098266 10 15.951475723672553 28.570541056495586 33.49790343771787 21.980079782113986 11 34.92517863053058 21.827575085034308 24.120979782372 19.126266502063103 12 23.976260786193674 24.483489932885906 30.927018216682644 20.613231064237773 13 29.68698830644821 21.424117691721868 25.439821578809724 23.449072423020198 14 21.478007533788553 21.925786354517513 26.92125411812342 29.674951993570513 15 23.736008744905863 30.44222584079808 22.949204933617622 22.872560480678427 16 22.63452193537058 27.53333573604707 27.29780588782445 22.534336440757897 17 22.337708645250835 28.4068100736333 27.136467940472244 22.119013340643626 18 22.335796872292164 26.520835282335675 29.488162590504004 21.65520525486816 19 24.135590932896967 26.2595861203789 27.458536506814283 22.146286439909854 20 24.473857346827042 26.34570290634101 27.672274049661304 21.508165697170647 21 23.446914916947446 25.95233238811944 27.240870046637028 23.359882648296086 22 24.11624221831916 27.143415270785432 26.81418551964202 21.926156991253386 23 22.750043324433296 27.113120478731155 27.691482493094927 22.445353703740622 24 23.808165470260064 26.2214906908026 27.90926556622564 22.061078272711693 25 23.143988945751676 26.62401446499309 27.732851575859165 22.499145013396067 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 259.0 1 259.0 2 538.0 3 817.0 4 817.0 5 817.0 6 2175.5 7 3534.0 8 3534.0 9 3534.0 10 4615.0 11 5696.0 12 5696.0 13 5696.0 14 8118.5 15 10541.0 16 10541.0 17 10541.0 18 18840.0 19 27139.0 20 27139.0 21 27139.0 22 43078.5 23 59018.0 24 59018.0 25 59018.0 26 86171.0 27 113324.0 28 113324.0 29 113324.0 30 145334.0 31 177344.0 32 177344.0 33 177344.0 34 202042.0 35 226740.0 36 226740.0 37 226740.0 38 246228.0 39 265716.0 40 265716.0 41 265716.0 42 285739.5 43 305763.0 44 305763.0 45 305763.0 46 327066.0 47 348369.0 48 348369.0 49 348369.0 50 339625.0 51 330881.0 52 330881.0 53 330881.0 54 298783.5 55 266686.0 56 266686.0 57 266686.0 58 232320.5 59 197955.0 60 197955.0 61 197955.0 62 165253.5 63 132552.0 64 132552.0 65 132552.0 66 103962.0 67 75372.0 68 75372.0 69 75372.0 70 56186.5 71 37001.0 72 37001.0 73 37001.0 74 26029.5 75 15058.0 76 15058.0 77 15058.0 78 10413.5 79 5769.0 80 5769.0 81 5769.0 82 3887.0 83 2005.0 84 2005.0 85 2005.0 86 1273.5 87 542.0 88 542.0 89 542.0 90 333.0 91 124.0 92 124.0 93 124.0 94 71.0 95 18.0 96 18.0 97 18.0 98 11.5 99 5.0 100 5.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.14201212470688912 2 0.014300897007470207 3 0.0 4 0.002568793832441029 5 0.08036107272830442 6 0.037611742531711186 7 0.023579226969421385 8 0.02403930944687351 9 0.02311914449196926 10 0.024000969240419168 11 0.028793495047212132 12 0.027911670298762226 13 0.017866536207724174 14 0.009355010374859868 15 0.0031055567228018407 16 0.002530453625986685 17 0.0012652268129933425 18 0.002147051561443248 19 0.0011502061936303114 20 0.0 21 0.0 22 7.284639226325305E-4 23 0.0 24 3.8340206454343715E-5 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2608228.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.12430699664189 #Duplication Level Percentage of deduplicated Percentage of total 1 78.27650267786403 49.411499856609495 2 13.711310025725712 17.31033886780087 3 3.982123455258312 7.541063504647622 4 1.5801020804994232 3.9897139526191263 5 0.7741284000481524 2.4433159389729386 6 0.4559628062904082 1.7269401697995648 7 0.29116281239253805 1.2865615528830556 8 0.17486067328221677 0.8830367057524917 9 0.1376315706112306 0.7819107764123984 >10 0.5623485778633688 5.971709510394123 >50 0.028803120518958114 1.2766528120427563 >100 0.020205179946335135 2.67677977032002 >500 0.0026115080883863852 1.2016388517931365 >1k 0.0020649133721788084 2.595921757609807 >5k 1.8219823872165958E-4 0.9029159723424773 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 9637 0.3694845696005104 No Hit TATCAACGCAGAGTACTTTTTTTTT 7030 0.26953165137403634 No Hit GGTATCAACGCAGAGTACTTTTTTT 6885 0.26397232143815647 No Hit CTGTAGGACGTGGAATATGGCAAGA 4709 0.18054403219350457 No Hit GTCCTACAGTGGACATTTCTAAATT 4484 0.17191748574127722 No Hit GTACATGGGAAGCAGTGGTATCAAC 4179 0.1602237227727024 No Hit CTTTAGGACGTGAAATATGGCGAGG 4005 0.15355252684964657 No Hit GTCCTAAAGTGTGTATTTCTCATTT 3819 0.14642124844913865 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 3281 0.1257942173767017 No Hit CCCATGTACTCTGCGTTGATACCAC 2678 0.10267507288473246 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 3.8340206454343715E-5 7 0.0 0.0 0.0 0.0 3.8340206454343715E-5 8 0.0 0.0 0.0 0.0 3.8340206454343715E-5 9 0.0 0.0 0.0 0.0 7.668041290868743E-5 10 0.0 0.0 0.0 0.0 7.668041290868743E-5 11 0.0 0.0 0.0 0.0 7.668041290868743E-5 12 0.0 0.0 0.0 0.0 1.1502061936303115E-4 13 0.0 0.0 0.0 0.0 1.1502061936303115E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGCGAGG 1200 0.0 11.31575 19 TAGGACC 770 0.0 10.862472 4 TTAGGAC 1660 0.0 10.7643175 3 GAACCGC 80 3.7714682E-4 10.687823 6 CGCCCTA 190 9.094947E-12 10.495286 16 AGGACCG 110 6.012442E-6 10.368921 5 TAGGACA 760 0.0 10.255031 4 CGCAACG 75 0.0026584854 10.128783 17 AGGACCT 1540 0.0 10.122043 5 GACGTGA 1455 0.0 10.119223 7 TTATACT 295 0.0 9.987951 4 TGGCGAG 2790 0.0 9.904154 18 CACCTTT 790 0.0 9.857467 14 AGGACGT 2710 0.0 9.855577 5 CTAGACA 300 0.0 9.8214855 4 GGACGTG 2700 0.0 9.746592 6 TGTAGGA 2785 0.0 9.728175 2 CTTATAC 285 0.0 9.6714115 3 ACCTTTT 750 0.0 9.622713 15 TATGGCG 2945 0.0 9.511856 16 >>END_MODULE