FastQCFastQC Report
Thu 26 May 2016
SRR1512644_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512644_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1663847
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT80110.4814745586583382No Hit
GGTATCAACGCAGAGTACTTTTTTT52130.31331005795605005No Hit
GTCCTACAGTGGACATTTCTAAATT48610.2921542665882139No Hit
TATCAACGCAGAGTACTTTTTTTTT48600.2920941649081917No Hit
GTCCTAAAGTGTGTATTTCTCATTT44780.26913532313968774No Hit
CTGTAGGACGTGGAATATGGCAAGA42440.25507153001447846No Hit
CTTTAGGACGTGAAATATGGCGAGG40070.24082743184920247No Hit
GTACTTTTTTTTTTTTTTTTTTTTT22870.13745254221091244No Hit
ACGCAGAGTACTTTTTTTTTTTTTT22760.13679142373066755No Hit
CTGAAGGACCTGGAATATGGCGAGA20140.12104478356483499No Hit
GTCCTACAGTGTGCATTTCTCATTT19400.11659725924318763No Hit
GTACATGGGAAGCAGTGGTATCAAC18990.1141330903622749No Hit
ATTTAGAAATGTCCACTGTAGGACG18120.10890424420033813No Hit
GTCCTTCAGTGTGCATTTCTCATTT18050.1084835324401823No Hit
GAGTACTTTTTTTTTTTTTTTTTTT17610.10583905851920278No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGTAC500.001514383713.2827173
TAGGACC6150.012.8044784
GTACCGT751.4986192E-512.6502076
TGTTCCG604.1374046E-412.6502075
GGACCGT550.003098617312.0751966
CCTAATA1157.2024704E-811.5501882
GGCGAGG12300.011.24986919
GGTATCA17300.010.8292391
CCGTGAG700.001544631310.8116679
GTCTAAG1001.6845705E-510.7895511
TAAGCGA2150.010.59151113
TTCCACC7000.010.56716811
CTAATAC1355.795664E-810.5418393
TGTAGGA25950.010.4199682
ATAGGAC2650.010.3827173
AAGCGAC2200.010.3536114
AGGACCT15400.010.3501685
GGACCTG15050.010.3387076
TGGCGAG26150.010.32685418
GTCCTAC23000.010.320441
Produced by FastQC (version 0.11.5)