FastQCFastQC Report
Thu 26 May 2016
SRR1512644_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512644_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1663847
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT98860.5941652087000788No Hit
GGTATCAACGCAGAGTACTTTTTTT73000.4387422641625101No Hit
TATCAACGCAGAGTACTTTTTTTTT72590.4362780952815974No Hit
CTGTAGGACGTGGAATATGGCAAGA50400.3029124673121988No Hit
GTCCTACAGTGGACATTTCTAAATT45770.27508538946189165No Hit
CTTTAGGACGTGAAATATGGCGAGG45660.27442427098164673No Hit
GTCCTAAAGTGTGTATTTCTCATTT43140.2592786476160368No Hit
ACGCAGAGTACTTTTTTTTTTTTTT32510.19539056175237268No Hit
GTACTTTTTTTTTTTTTTTTTTTTT26170.15728609661825876No Hit
CTGAAGGACCTGGAATATGGCGAGA25720.154581521017257No Hit
GAGTACTTTTTTTTTTTTTTTTTTT22810.13709193213077886No Hit
GTACATGGGAAGCAGTGGTATCAAC21400.12861759524763994No Hit
TTTCTAAATTTTCCACCTTTTTCAG21100.1268145448469721No Hit
ATTTAGAAATGTCCACTGTAGGACG20970.1260332230066827No Hit
GTCCTACAGTGTGCATTTCTCATTT18230.10956536268058302No Hit
GAATATGGCAAGAAAACTGAAAATC17240.10361529635837911No Hit
CTGTAGGACCTGGAATATGGCGAGA16950.10187234763773352No Hit
GTCCTTCAGTGTGCATTTCTCATTT16830.10115112747746638No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC6800.012.4404194
TGTCGAG1155.3641998E-912.38520818
TCCAACG951.0461044E-611.99409818
GGTATCA21500.011.6379481
ATTGGAC1001.9180789E-611.4057043
GTGTCGA752.0805749E-411.39610513
ATGTCGA1251.845001E-811.39439317
ACCTTTT6750.011.39439215
GTCGAAA600.005897510311.07788215
TCGAAAC600.005897510311.07788216
GGCGAGG11550.010.85180319
TATACTG2300.010.7448265
TATGTCG1252.2550012E-710.63476716
TAGAAAT7900.010.4675754
GTCGAGG1106.0804723E-610.35853919
AAATTTC1106.0804723E-610.35853916
TCGAACT1201.5331134E-610.28660519
CCACCTT6950.010.24829613
AGGACCT16100.010.2134895
GTATAGG1409.480755E-810.1934771
Produced by FastQC (version 0.11.5)