FastQCFastQC Report
Thu 26 May 2016
SRR1512643_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512643_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1796158
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT69200.3852667749719123No Hit
GGTATCAACGCAGAGTACTTTTTTT45140.2513141939628919No Hit
TATCAACGCAGAGTACTTTTTTTTT41160.22915578696306227No Hit
GTCCTACAGTGGACATTTCTAAATT37490.2087232860360837No Hit
GTCCTAAAGTGTGTATTTCTCATTT36400.2026547775863816No Hit
CTGTAGGACGTGGAATATGGCAAGA31680.17637646576748817No Hit
CTTTAGGACGTGAAATATGGCGAGG28130.15661205751387128No Hit
GTACATGGGAAGCAGTGGTATCAAC24240.1349547200190629No Hit
GTCCTACAGTGTGCATTTCTCATTT23030.12821811889599913No Hit
GTACTTTTTTTTTTTTTTTTTTTTT20100.11190552278808434No Hit
ACGCAGAGTACTTTTTTTTTTTTTT20030.11151580206195669No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACATCG500.001516036213.280897
GTCTTAC1950.011.5783891
GTTCCGG600.00460299711.4525381
TAGGACC6250.011.2318384
GTCCTAT1905.456968E-1210.84977151
TGTAGGA20650.010.841242
GTATAGA2100.010.7513611
GTACTAG1201.0000895E-610.6344991
GTCCTAG2600.010.5715731
TTAGGAC12950.010.401733
TAGGACT3200.010.3756944
GTAGGAC20100.010.3355853
GGCGAGG9750.010.30701619
GTCCTAC20600.010.0547241
AGGACGT21200.010.0233135
GGACGTG20600.09.9929036
TATCGCG951.6681601E-49.9856317
ACGTGAA12650.09.9442768
TATACTC2107.2759576E-129.938085
GCGCCAC1151.04271185E-59.89990913
Produced by FastQC (version 0.11.5)