FastQCFastQC Report
Thu 26 May 2016
SRR1512643_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512643_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1796158
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT84230.4689453823104649No Hit
GGTATCAACGCAGAGTACTTTTTTT60010.3341020110702956No Hit
TATCAACGCAGAGTACTTTTTTTTT59300.3301491294195722No Hit
CTGTAGGACGTGGAATATGGCAAGA36970.20582821778484967No Hit
GTCCTACAGTGGACATTTCTAAATT36420.2027661263652752No Hit
GTCCTAAAGTGTGTATTTCTCATTT33860.18851348266689233No Hit
CTTTAGGACGTGAAATATGGCGAGG33560.18684325098348809No Hit
ACGCAGAGTACTTTTTTTTTTTTTT27350.15226945513702023No Hit
GTACATGGGAAGCAGTGGTATCAAC24760.13784978827029692No Hit
GTACTTTTTTTTTTTTTTTTTTTTT22700.12638086404425447No Hit
GTCCTACAGTGTGCATTTCTCATTT21760.1211474714362545No Hit
GAGTACTTTTTTTTTTTTTTTTTTT18620.10366571314995673No Hit
CTGAAGGACCTGGAATATGGCGAGA18220.10143873757208442No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC6450.013.4110044
TGGACCG550.003053971812.0987125
GGACTGT1900.010.9997426
GGACCGT951.3607638E-510.9997426
GGCGAGG9350.010.96720119
CGTGCGC700.001493623610.85537610
GTCTAGA1951.8189894E-1210.7377351
CCACCTT6650.010.71045513
AGGACCT13700.010.6857245
GGTATCA22100.010.6372481
TGGCGAG20450.010.539700518
TTAGGAC13600.010.4841183
ATTTAGA7450.010.4756791
GTCGAGG1106.0857383E-610.35791119
GGACGTG21400.010.3432156
AATGTCC6800.010.3365548
TATGTCG1201.5334645E-610.28655516
GGACCTG13700.010.2625326
TAATACT2250.010.1393084
ACCGTCT750.00265149110.1316848
Produced by FastQC (version 0.11.5)