FastQCFastQC Report
Thu 26 May 2016
SRR1512642_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512642_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1519256
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT60360.39729973092092447No Hit
GGTATCAACGCAGAGTACTTTTTTT39910.2626943714555019No Hit
GTCCTACAGTGGACATTTCTAAATT35870.23610240802076804No Hit
TATCAACGCAGAGTACTTTTTTTTT35170.23149488960385872No Hit
GTCCTAAAGTGTGTATTTCTCATTT33470.22030520201993609No Hit
CTGTAGGACGTGGAATATGGCAAGA31090.20463963940244434No Hit
CTTTAGGACGTGAAATATGGCGAGG28020.18443238005971344No Hit
ACGCAGAGTACTTTTTTTTTTTTTT17840.11742589793951777No Hit
GTCCTACAGTGTGCATTTCTCATTT17830.11736007624784763No Hit
GTACTTTTTTTTTTTTTTTTTTTTT17340.1141348133560111No Hit
CTGAAGGACCTGGAATATGGCGAGA15500.10202362208870656No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCCG250.00615821218.9329
TTACACG456.831187E-414.7579994
TAGGACC6100.013.6865764
GGTATCA12900.012.7715241
GATCAAC1850.012.7222131
CGCCCTA853.9922434E-612.28050616
GGCGAGG8750.011.59239519
GCACCGT600.00593439311.06856
AGGACCT12100.010.8986185
GTACCGT700.001508115310.8426126
AGGACCG803.8174936E-410.6731965
TACACGT803.8174936E-410.6731965
GTATAGA1751.3096724E-1010.6472821
GTCCTAC19000.010.6325331
GTCTAAG1201.0125641E-610.6239311
TCCTACA21450.010.5712642
TGCGCCA909.685916E-510.53689912
ACTATCG1355.8395017E-810.53620211
GACGTGA11450.010.5230167
GGACCTG11450.010.5230166
Produced by FastQC (version 0.11.5)