FastQCFastQC Report
Thu 26 May 2016
SRR1512642_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512642_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1519256
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT76460.503272654509839No Hit
GGTATCAACGCAGAGTACTTTTTTT56820.37399885206969724No Hit
TATCAACGCAGAGTACTTTTTTTTT56130.36945715534445805No Hit
CTGTAGGACGTGGAATATGGCAAGA33330.2193836983365542No Hit
CTTTAGGACGTGAAATATGGCGAGG32510.21398631961960327No Hit
GTCCTAAAGTGTGTATTTCTCATTT30280.19930808237716355No Hit
GTCCTACAGTGGACATTTCTAAATT29050.19121201430173715No Hit
ACGCAGAGTACTTTTTTTTTTTTTT26510.1744933046175233No Hit
GTACTTTTTTTTTTTTTTTTTTTTT19420.1278257252233988No Hit
CTGAAGGACCTGGAATATGGCGAGA19150.12604853954830522No Hit
GAGTACTTTTTTTTTTTTTTTTTTT17770.11696514609782682No Hit
TTTCTAAATTTTCCACCTTTTTCAG15230.10024643641361299No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC5750.014.2145834
TCGCAGT500.001502283213.29675114
CGAACCG701.0926883E-412.21210612
AAGGCGA550.003077043212.08636417
GAACCGG658.0374785E-411.68983613
GGTATCA17400.011.5366691
GATATAC3400.011.4723431
AGGACCT12200.011.3739385
AATCCCG1701.2732926E-1111.1722719
GGCGAGG8600.011.15278219
ACTGTTC4050.011.023458
CCACCTT5700.010.99767513
GGACCTG12600.010.6301056
TAAGACT1701.382432E-1010.6220464
TAGAAAT5700.010.5043514
AAATGTC5650.010.4236057
CACCTTT6500.010.37438814
AATGCCG1106.070939E-610.3597418
TTAGGAC11400.010.3369333
TTTAGAA6500.010.2360192