FastQCFastQC Report
Thu 26 May 2016
SRR1512641_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512641_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2487609
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT72010.2894747526641044No Hit
GGTATCAACGCAGAGTACTTTTTTT44530.17900723144191874No Hit
TATCAACGCAGAGTACTTTTTTTTT40900.16441490604029813No Hit
GTCCTACAGTGGACATTTCTAAATT39110.15721924144831442No Hit
GTCCTAAAGTGTGTATTTCTCATTT35850.14411428805732734No Hit
CTGTAGGACGTGGAATATGGCAAGA33930.13639603329944536No Hit
GTACATGGGAAGCAGTGGTATCAAC31890.1281953876191958No Hit
CTTTAGGACGTGAAATATGGCGAGG28880.11609541531647458No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC7850.012.2061554
GGTGCGA701.1094535E-412.1928411
GCGTGCA1002.0255247E-611.3547469
TCTATAC1855.456968E-1210.7692013
GTTTAGG2750.010.7130021
GTAGGAC23000.010.4358693
GATATAC5100.010.3979141
TGTAGGA23950.010.3784312
GTGTAGG4450.010.3719521
CGTGCCG1106.179869E-610.34502110
GACTGTC2300.010.3121237
TAGAAAT6950.010.2377294
GGACGTG24000.010.1194276
AGGACGT24600.010.1045
TAGGACT4150.010.0584664
GTCCTAC21850.09.9326111
TCCTACA24800.09.9079552
AGGACCT15650.09.8809895
CCCTATA1252.6161451E-69.866642
GGCGAGG11250.09.85548419
Produced by FastQC (version 0.11.5)