FastQCFastQC Report
Thu 26 May 2016
SRR1512641_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512641_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2487609
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT92800.37304897996429504No Hit
GGTATCAACGCAGAGTACTTTTTTT60650.24380841201330272No Hit
TATCAACGCAGAGTACTTTTTTTTT59330.2385021118672589No Hit
GTCCTACAGTGGACATTTCTAAATT39670.15947039908603No Hit
CTGTAGGACGTGGAATATGGCAAGA38700.1555710724635584No Hit
GTCCTAAAGTGTGTATTTCTCATTT37960.15259632844229137No Hit
GTACATGGGAAGCAGTGGTATCAAC35900.1443152842749805No Hit
CTTTAGGACGTGAAATATGGCGAGG34830.14001396521720255No Hit
ACGCAGAGTACTTTTTTTTTTTTTT30290.12176350865429414No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC7450.012.6314084
GGACCGC855.327216E-511.1761396
GTCGTAC600.005797283311.1037721
GCGACTA1201.2857527E-711.077435516
GGCGAGG8700.010.6952419
AACACCG803.7503464E-410.694285
AAGACCG1002.383223E-510.456635
TAGGACT3200.010.3965894
GTCCTAA12200.010.3756551
ACTATCG2300.010.32474311
GCACCGT1752.3646862E-1010.3139796
TAAGCGA1201.5314963E-610.28805213
GTCCTAG3300.010.0943371
GTCCTAC20700.010.0692591
GTATTAG2105.456968E-129.9707351
TGTAGGA25400.09.9563332
TGGACCG1151.0192209E-59.9193335
CGACTAA1252.5895315E-69.87454517
ACTGTTC7600.09.8737148
GTAGGAC23700.09.8662213
Produced by FastQC (version 0.11.5)