FastQCFastQC Report
Thu 26 May 2016
SRR1512640_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512640_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2452889
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT103650.42256294516384557No Hit
GTCCTAAAGTGTGTATTTCTCATTT96470.3932913393145797No Hit
CTGTAGGACGTGGAATATGGCAAGA90580.36927883813739637No Hit
CTTTAGGACGTGAAATATGGCGAGG78910.3217022865690213No Hit
GTATCAACGCAGAGTACTTTTTTTT69130.28183093486904626No Hit
GTCCTACAGTGTGCATTTCTCATTT46510.18961314596787707No Hit
CTGAAGGACCTGGAATATGGCGAGA42740.17424351448434885No Hit
GGTATCAACGCAGAGTACTTTTTTT42660.17391736845817318No Hit
TATCAACGCAGAGTACTTTTTTTTT39230.15993385758589157No Hit
GTCCTTCAGTGTGCATTTCTCATTT38690.15773237190920583No Hit
ATTTAGAAATGTCCACTGTAGGACG38580.1572839211232143No Hit
TTTCTAAATTTTCCACCTTTTTCAG34520.14073201029479931No Hit
GAATATGGCAAGAAAACTGAAAATC31940.13021380095063412No Hit
CTGTAGGACCTGGAATATGGCGAGA30710.1251993057981833No Hit
GGAATATGGCGAGAAAACTGAAAAT28000.11415110916148263No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC14500.013.1501234
GGCGAGG26950.012.02829919
CACCTTT13100.011.95490214
ACCTTTT13300.011.91785715
CCACCTT12850.011.88666713
CCAACGA1603.6379788E-1211.84805119
TGTCGAG1603.6379788E-1211.84489618
GTCTAGC1251.1221346E-811.7847531
AATGTCC13600.011.5446798
GGACGGG1451.2478267E-911.1219956
AAATGTC13750.011.107727
CGATAAG600.00595294911.064312
TAGAAAT15000.010.8777484
ATGTAGG4250.010.8604581
CTGCGCA700.001545327410.8114639
ATTTAGG3550.010.7888571
GAAATGT14200.010.7557156
TAGGACT5500.010.69376854
TTCCACC13800.010.64978211
GTGTAGG5350.010.6466621
Produced by FastQC (version 0.11.5)