FastQCFastQC Report
Thu 26 May 2016
SRR1512640_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512640_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2452889
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGA105180.42880048791445513No Hit
GTCCTACAGTGGACATTTCTAAATT101780.4149392818019894No Hit
GTCCTAAAGTGTGTATTTCTCATTT95490.3892960504939278No Hit
CTTTAGGACGTGAAATATGGCGAGG92350.3764948189665329No Hit
GTATCAACGCAGAGTACTTTTTTTT85530.3486908702350575No Hit
GGTATCAACGCAGAGTACTTTTTTT58930.24024731653164902No Hit
TATCAACGCAGAGTACTTTTTTTTT56980.2322975071436172No Hit
CTGAAGGACCTGGAATATGGCGAGA53060.2163163518610096No Hit
GTCCTACAGTGTGCATTTCTCATTT45070.1837425174967151No Hit
ATTTAGAAATGTCCACTGTAGGACG43740.1783203398115447No Hit
TTTCTAAATTTTCCACCTTTTTCAG43550.17754574299937748No Hit
CTGTAGGACCTGGAATATGGCGAGA36480.1487225879361031No Hit
GAATATGGCAAGAAAACTGAAAATC36080.1470918578052248No Hit
GTCCTTCAGTGTGCATTTCTCATTT35440.14448268959581945No Hit
GGAATATGGCGAGAAAACTGAAAAT32490.13245605488059184No Hit
ACGCAGAGTACTTTTTTTTTTTTTT29090.11859484876812607No Hit
ATTCCAGGTCCTTCAGTGTGCATTT28950.11802409322231866No Hit
GCCATATTCCACGTCCTACAGTGGA28320.11545569326618531No Hit
ACAGTGGACATTTCTAAATTTTCCA27670.11280575680350803No Hit
TTCCAGGTCCTTCAGTGTGCATTTC26450.10783202990432915No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACCGT400.005282471414.2490986
TAGGACC15150.013.3634934
TGCACCG905.3746953E-712.6733595
TATGTCG2000.012.34468716
ACCTTTT12800.012.24057715
CACCTTT13100.011.81600714
CCACCTT12900.011.78027813
AATGTCC14150.011.6131588
GATATAC7050.011.4718911
AAATGTC13600.011.3843797
TAGAAAT14600.011.32794
GAAATGT13900.011.2079236
GCACCGT855.3289747E-511.1757626
AGGACCT33150.011.12503055
ATGTCCA14950.011.1187919
TGTCCAC15100.011.00833910
GGTATCA22150.010.7821461
GGACCTG32300.010.6463856
AATCCCG3750.010.63520519
CTTTTTC17100.010.55101417
Produced by FastQC (version 0.11.5)