##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512640_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2452889 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.5948552910466 33.0 31.0 34.0 29.0 34.0 2 31.74783816144962 34.0 31.0 34.0 29.0 34.0 3 31.84533992365737 34.0 31.0 34.0 28.0 34.0 4 35.296209082433 37.0 35.0 37.0 33.0 37.0 5 35.06801123083841 37.0 35.0 37.0 32.0 37.0 6 34.9264879087476 37.0 35.0 37.0 32.0 37.0 7 34.918567452501925 37.0 35.0 37.0 32.0 37.0 8 34.902750593280004 37.0 35.0 37.0 32.0 37.0 9 36.49556176410755 39.0 37.0 39.0 32.0 39.0 10 36.286176830667834 39.0 37.0 39.0 32.0 39.0 11 36.394013345080026 39.0 37.0 39.0 32.0 39.0 12 36.340406353487666 39.0 37.0 39.0 32.0 39.0 13 36.339537582010436 39.0 37.0 39.0 32.0 39.0 14 37.573743043407184 40.0 38.0 41.0 32.0 41.0 15 37.57318207224216 40.0 38.0 41.0 32.0 41.0 16 37.54049041762591 40.0 38.0 41.0 32.0 41.0 17 37.51928399532144 40.0 38.0 41.0 32.0 41.0 18 37.4574638314249 40.0 38.0 41.0 32.0 41.0 19 37.48389755916391 40.0 38.0 41.0 32.0 41.0 20 37.450380755101435 40.0 38.0 41.0 32.0 41.0 21 37.395770049113516 40.0 38.0 41.0 32.0 41.0 22 37.3042245287088 40.0 38.0 41.0 32.0 41.0 23 37.23902589966362 40.0 38.0 41.0 32.0 41.0 24 37.218857029404916 40.0 38.0 41.0 32.0 41.0 25 37.1503671792731 40.0 38.0 41.0 32.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.585739799266694 1101 2 0.5324603720127534 1101 3 0.5681842218318316 1101 4 0.45504507413465944 1101 5 0.5408332278408423 1101 6 0.7432972212238482 1101 7 0.6874828351620152 1101 8 0.7588988812805013 1101 9 0.7873758806205657 1101 10 0.9233017159852182 1101 11 0.7872723385615004 1101 12 0.7667896960721308 1101 13 0.7813019467342102 1101 14 1.0329834996850877 1101 15 1.0114453575199107 1101 16 1.005795740281478 1101 17 1.0139099728136642 1101 18 1.083255056831831 1101 19 0.941180452777111 1101 20 0.9139751050532041 1101 21 1.099476393205478 1101 22 1.1332143128135854 1101 23 0.9994448260769815 1101 24 1.0048789334824235 1101 25 1.0661279303351208 1102 1 0.47114080781496526 1102 2 0.4521822625586047 1102 3 0.5162573515079991 1102 4 0.4827012700698319 1102 5 0.4250010266190145 1102 6 0.5907629813504585 1102 7 0.4681207504507725 1102 8 0.4309636399493115 1102 9 0.5277495057786865 1102 10 0.7359045277814857 1102 11 0.54983806720643 1102 12 0.5149784358405896 1102 13 0.6404421635891069 1102 14 0.7334303410230874 1102 15 0.843685931450814 1102 16 0.8901078300978611 1102 17 0.8902481215206421 1102 18 0.809523967111879 1102 19 0.8043271389756512 1102 20 0.9066747584710981 1102 21 0.8218248060749573 1102 22 0.8587966884609415 1102 23 0.8334929377433582 1102 24 0.7687643756156888 1102 25 0.7368398275743786 1103 1 0.3736995058726045 1103 2 0.438045633277806 1103 3 0.40153809152031883 1103 4 0.33355395010122635 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-1.6623887960491786 2314 20 -1.829311279923317 2314 21 -1.7127031842593112 2314 22 -1.661096720050267 2314 23 -1.6774108077258347 2314 24 -1.7691058083016173 2314 25 -1.7081091588667547 2315 1 -0.7026955534242916 2315 2 -0.514152005687567 2315 3 -0.6669963887071013 2315 4 -0.4948746523241283 2315 5 -0.5328924173704763 2315 6 -0.5424113611776136 2315 7 -0.8576543560329313 2315 8 -0.9603412046250028 2315 9 -1.03097163448124 2315 10 -0.8500558144155548 2315 11 -0.9942574523527057 2315 12 -1.161964003805366 2315 13 -1.1694139434181992 2315 14 -1.3371442282352533 2315 15 -1.4736608283072528 2315 16 -1.3321619470519295 2315 17 -1.3303815367889342 2315 18 -1.317768948341424 2315 19 -1.2451969334138724 2315 20 -1.083301574809468 2315 21 -1.1126923593246332 2315 22 -1.245678280333955 2315 23 -1.3087426479647277 2315 24 -1.362892669070554 2315 25 -1.560974033820095 2316 1 -1.368462638788973 2316 2 -1.1851542910196713 2316 3 -1.1148667684918863 2316 4 -0.9991907802541462 2316 5 -1.1472089351152022 2316 6 -4.549823624239384 2316 7 -2.402440653710478 2316 8 -1.7750649341500448 2316 9 -1.7506628179482888 2316 10 -1.494508016596825 2316 11 -1.5778380635474676 2316 12 -1.6015027593109252 2316 13 -1.5554903635264878 2316 14 -1.8865166901742398 2316 15 -1.83715737601797 2316 16 -1.8397208243994498 2316 17 -1.8931068297370004 2316 18 -2.0680731946728557 2316 19 -2.1078775885292984 2316 20 -2.140865871391142 2316 21 -2.3820626512565255 2316 22 -2.2667021327142436 2316 23 -2.4348307023648204 2316 24 -2.3805555552285824 2316 25 -2.385949774505633 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 25427.0 3 1.0 4 505.0 5 4260.0 6 5515.0 7 4302.0 8 3371.0 9 2984.0 10 2905.0 11 2642.0 12 2611.0 13 2402.0 14 2421.0 15 2461.0 16 2509.0 17 2611.0 18 2936.0 19 3289.0 20 4056.0 21 5426.0 22 6860.0 23 8461.0 24 10777.0 25 12575.0 26 14165.0 27 16286.0 28 18851.0 29 22736.0 30 29357.0 31 38336.0 32 49083.0 33 65621.0 34 87242.0 35 119557.0 36 178968.0 37 330039.0 38 1361341.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.09942705764375 20.10720620371792 13.605584391403905 25.187782347234428 2 14.12735899062683 20.80694563822205 42.73489601766664 22.330799353484483 3 18.561541105202885 25.730883052596347 30.564734074799144 25.142841767401624 4 12.41553072694027 17.796450201095475 38.84540823502792 30.942610836936336 5 12.149968638528536 38.93388952845161 36.46757607452319 12.448565758496674 6 30.741860675682396 38.02187888225357 18.353643068003013 12.88261737406102 7 27.18597803042074 31.858132810050037 23.082901987554894 17.87298717197433 8 23.90133876303988 39.29146570676241 20.735799728324366 16.071395801873347 9 26.31275031104735 14.747212603871057 21.06301323832201 37.877023846759585 10 15.415338427302064 29.06645145688675 35.069189427697225 20.44902068811397 11 33.11039053461003 21.581624184508545 26.930049114026676 18.37793616685474 12 24.198263332098993 24.345323834894202 32.888372677251546 18.568040155755263 13 29.44580164437991 22.63034726240472 26.054528038989496 21.86932305422588 14 19.643048630373524 23.980603464971708 27.965349467484142 28.410998437170633 15 21.597497076824475 34.396684295412776 23.093494324065603 20.912324303697144 16 20.17904311654722 28.18298307619362 30.782565515486887 20.855408291772275 17 20.413762737707692 30.154860356604857 29.126507681864606 20.304869223822852 18 20.35559755694343 26.924053817041464 33.15465529802526 19.565693327989845 19 23.44882160057174 26.367780318574923 28.56855728881498 21.614840792038358 20 23.666582548170748 27.86550879391607 29.191985450625772 19.275923207287406 21 21.61410483719402 26.893593635912595 28.03763235923028 23.454669167663113 22 22.97509293165009 29.704779446837552 27.82070694098932 19.499420680523045 23 20.419676552832193 29.450252335103627 29.556168257104176 20.573902854960007 24 23.116139016538874 27.02883295119875 30.117070163823218 19.737957868439164 25 21.59021464077665 28.358560048987137 29.691355784953988 20.359869525282228 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 260.0 1 260.0 2 526.0 3 792.0 4 792.0 5 792.0 6 1961.5 7 3131.0 8 3131.0 9 3131.0 10 4325.5 11 5520.0 12 5520.0 13 5520.0 14 8409.5 15 11299.0 16 11299.0 17 11299.0 18 21002.5 19 30706.0 20 30706.0 21 30706.0 22 50549.5 23 70393.0 24 70393.0 25 70393.0 26 106654.5 27 142916.0 28 142916.0 29 142916.0 30 187550.5 31 232185.0 32 232185.0 33 232185.0 34 253897.0 35 275609.0 36 275609.0 37 275609.0 38 284044.5 39 292480.0 40 292480.0 41 292480.0 42 301421.0 43 310362.0 44 310362.0 45 310362.0 46 317360.0 47 324358.0 48 324358.0 49 324358.0 50 300954.0 51 277550.0 52 277550.0 53 277550.0 54 235096.5 55 192643.0 56 192643.0 57 192643.0 58 161223.0 59 129803.0 60 129803.0 61 129803.0 62 105166.5 63 80530.0 64 80530.0 65 80530.0 66 61267.0 67 42004.0 68 42004.0 69 42004.0 70 30715.5 71 19427.0 72 19427.0 73 19427.0 74 13372.5 75 7318.0 76 7318.0 77 7318.0 78 4895.5 79 2473.0 80 2473.0 81 2473.0 82 1651.0 83 829.0 84 829.0 85 829.0 86 534.5 87 240.0 88 240.0 89 240.0 90 142.5 91 45.0 92 45.0 93 45.0 94 29.5 95 14.0 96 14.0 97 14.0 98 8.0 99 2.0 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1539816926081857 2 0.014554266418089037 3 0.0 4 0.003016850742124898 5 0.09906685545085815 6 0.041868996110300954 7 0.027722412224931502 8 0.02853777729037066 9 0.028130094757651082 10 0.028863923316546327 11 0.034245332748444796 12 0.03412302798862892 13 0.021851783753769533 14 0.011659720435780013 15 0.003139155501940773 16 0.0029353142355809823 17 0.001386120611246575 18 0.0024460951963174854 19 0.0011415110916148264 20 0.0 21 0.0 22 4.484507859915389E-4 23 0.0 24 4.076825327195809E-5 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2452889.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.942124377772366 #Duplication Level Percentage of deduplicated Percentage of total 1 83.04657673872356 53.10174538975679 2 11.460513309343384 14.656191349183006 3 2.8116102085501873 5.393409889707917 4 1.0338433311790418 2.6442455547752317 5 0.465416304745079 1.4879853622726524 6 0.26867938714237727 1.0307958474240926 7 0.16962311548759185 0.759224364349699 8 0.119988675381455 0.6137864644132119 9 0.08779761937575928 0.5052569668377406 >10 0.46245928193503094 5.350870337041138 >50 0.03645991241983171 1.6181456646444357 >100 0.028419273803263263 3.7792690883299436 >500 0.00497630866103613 2.251030940840552 >1k 0.003126142620391365 4.159267155402537 >5k 3.827929739254733E-4 1.8044962420635742 >10k+ 1.2759765797515778E-4 0.8442793829576283 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTGTAGGACGTGGAATATGGCAAGA 10518 0.42880048791445513 No Hit GTCCTACAGTGGACATTTCTAAATT 10178 0.4149392818019894 No Hit GTCCTAAAGTGTGTATTTCTCATTT 9549 0.3892960504939278 No Hit CTTTAGGACGTGAAATATGGCGAGG 9235 0.3764948189665329 No Hit GTATCAACGCAGAGTACTTTTTTTT 8553 0.3486908702350575 No Hit GGTATCAACGCAGAGTACTTTTTTT 5893 0.24024731653164902 No Hit TATCAACGCAGAGTACTTTTTTTTT 5698 0.2322975071436172 No Hit CTGAAGGACCTGGAATATGGCGAGA 5306 0.2163163518610096 No Hit GTCCTACAGTGTGCATTTCTCATTT 4507 0.1837425174967151 No Hit ATTTAGAAATGTCCACTGTAGGACG 4374 0.1783203398115447 No Hit TTTCTAAATTTTCCACCTTTTTCAG 4355 0.17754574299937748 No Hit CTGTAGGACCTGGAATATGGCGAGA 3648 0.1487225879361031 No Hit GAATATGGCAAGAAAACTGAAAATC 3608 0.1470918578052248 No Hit GTCCTTCAGTGTGCATTTCTCATTT 3544 0.14448268959581945 No Hit GGAATATGGCGAGAAAACTGAAAAT 3249 0.13245605488059184 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 2909 0.11859484876812607 No Hit ATTCCAGGTCCTTCAGTGTGCATTT 2895 0.11802409322231866 No Hit GCCATATTCCACGTCCTACAGTGGA 2832 0.11545569326618531 No Hit ACAGTGGACATTTCTAAATTTTCCA 2767 0.11280575680350803 No Hit TTCCAGGTCCTTCAGTGTGCATTTC 2645 0.10783202990432915 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 4.076825327195809E-5 0.0 5 0.0 0.0 0.0 4.076825327195809E-5 0.0 6 0.0 0.0 0.0 4.076825327195809E-5 0.0 7 0.0 0.0 0.0 4.076825327195809E-5 0.0 8 0.0 0.0 0.0 4.076825327195809E-5 0.0 9 0.0 0.0 0.0 4.076825327195809E-5 0.0 10 0.0 0.0 0.0 4.076825327195809E-5 0.0 11 0.0 0.0 0.0 4.076825327195809E-5 0.0 12 0.0 0.0 0.0 4.076825327195809E-5 0.0 13 0.0 0.0 0.0 8.153650654391617E-5 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGACCGT 40 0.0052824714 14.249098 6 TAGGACC 1515 0.0 13.363493 4 TGCACCG 90 5.3746953E-7 12.673359 5 TATGTCG 200 0.0 12.344687 16 ACCTTTT 1280 0.0 12.240577 15 CACCTTT 1310 0.0 11.816007 14 CCACCTT 1290 0.0 11.780278 13 AATGTCC 1415 0.0 11.613158 8 GATATAC 705 0.0 11.471891 1 AAATGTC 1360 0.0 11.384379 7 TAGAAAT 1460 0.0 11.3279 4 GAAATGT 1390 0.0 11.207923 6 GCACCGT 85 5.3289747E-5 11.175762 6 AGGACCT 3315 0.0 11.1250305 5 ATGTCCA 1495 0.0 11.118791 9 TGTCCAC 1510 0.0 11.008339 10 GGTATCA 2215 0.0 10.782146 1 GGACCTG 3230 0.0 10.646385 6 AATCCCG 375 0.0 10.635205 19 CTTTTTC 1710 0.0 10.551014 17 >>END_MODULE