FastQCFastQC Report
Thu 26 May 2016
SRR1512639_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512639_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2143619
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT68910.3214657082252023No Hit
GTCCTACAGTGGACATTTCTAAATT56650.26427270890955906No Hit
GTCCTAAAGTGTGTATTTCTCATTT50190.2341367565784778No Hit
CTGTAGGACGTGGAATATGGCAAGA49190.22947174847769122No Hit
GGTATCAACGCAGAGTACTTTTTTT40940.19098543164620205No Hit
CTTTAGGACGTGAAATATGGCGAGG40200.18753332565161998No Hit
TATCAACGCAGAGTACTTTTTTTTT37790.17629065612872438No Hit
GTCCTACAGTGTGCATTTCTCATTT30880.14405545015228918No Hit
CTGAAGGACCTGGAATATGGCGAGA22290.10398303056653257No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC9400.014.0289654
GGTATCA12350.013.0258721
GTGTAGG3700.011.9300081
TCCAACG1201.03827915E-811.8465218
GTCCTAT3000.011.7709421
TATGTCG907.5511725E-611.59901716
GGCGAGG13700.011.34791119
TGTCCAC10050.011.13295310
TCTAGGA3550.010.9570632
AGGACCT19450.010.9261485
CCGTCCA700.00153900210.8166689
CACCTTT7950.010.74326314
AATGTCC9000.010.7245238
GGACCTG19400.010.6119866
TTTAGAA9700.010.5630832
GACGTGG16250.010.5089137
AAATGTC8950.010.4942367
CCCTATA1002.4354498E-510.4359342
GTCCTAG3950.010.4299471
TGTAGGA34500.010.4221842
Produced by FastQC (version 0.11.5)