FastQCFastQC Report
Thu 26 May 2016
SRR1512639_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512639_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2143619
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT86630.4041296517711403No Hit
TATCAACGCAGAGTACTTTTTTTTT59040.2754220782704389No Hit
GGTATCAACGCAGAGTACTTTTTTT58730.2739759257591951No Hit
CTGTAGGACGTGGAATATGGCAAGA53530.24971788363510491No Hit
GTCCTACAGTGGACATTTCTAAATT51290.23926826548934305No Hit
CTTTAGGACGTGAAATATGGCGAGG47600.22205438559744062No Hit
GTCCTAAAGTGTGTATTTCTCATTT46230.21566332449936298No Hit
GTCCTACAGTGTGCATTTCTCATTT28810.134398883383661No Hit
ACGCAGAGTACTTTTTTTTTTTTTT28110.1311333777131104No Hit
CTGAAGGACCTGGAATATGGCGAGA27650.12898747398674856No Hit
CTGTAGGACCTGGAATATGGCGAGA24670.1150857498464046No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCGCC604.0973088E-412.6651429
TCCAACG1052.00871E-812.66041618
GGTATCA17900.012.6513141
TAGGACC9900.012.0979224
GGCGAGG12900.011.70352419
CCAACGA907.5052685E-611.6053819
CTAGGAC3050.011.2193583
ACTGCGC600.005882157511.0819998
CACCTTT7550.010.94314914
GATATAC5100.010.8210541
TGTAGGA31900.010.7880772
AGGACGT30300.010.6035075
CCACCTT8000.010.56501313
ATAGGAC3700.010.5328873
GTAGGAC30700.010.4960683
TAGGACA9800.010.4754614
ATTTAGA7450.010.4729561
GGACGTG30300.010.4403126
CTGTAGG32050.010.331491
TTAGACT1752.3283064E-1010.3202694
Produced by FastQC (version 0.11.5)